LOCUS       sequence-108498-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This pFTV1 vector contains a theophylline binding riboswitch, 
            controlling the translation initaition rate of mRFP1 gene. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 241 to 275)
  AUTHORS   Espah Borujeni A, Mishler DM, Wang J, Huso W, Salis HM
  TITLE     Automated physics-based design of synthetic riboswitches from 
            diverse RNA aptamers.
  JOURNAL   Nucleic Acids Res. 2015 Nov 30. pii: gkv1289.
  PUBMED    26621913
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Nov 30. pii: gkv1289."
COMMENT     Sequence Label: AEB-Theo45
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(241..275)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     promoter        328..430
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(497..516)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ctttcagttt agcggtctgg gtaccttcgt acggacgacc ttcaccttca ccttcgattt
       61 cgaactcgtg accgttaacg gaaccttcca tacgaacttt gaaacgcatg aactctttga
      121 taacgtcttc agagctcgcg gtgcgacgac gcatcggatg acctccttta gtagggagga
      181 gctgccaagg gcatcaagac gatgctggta tctccgtgaa cttctggact cgttctagaa
      241 gctagcactg tacctaggac tgagctagcc gtcaacggat cccctaggga attccgcgct
      301 cgaggatatc tggcgaaaat gagacgttga tcggcacgta agaggttcca actttcacca
      361 taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt caggagctaa
      421 ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca
      481 tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt
      541 tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc
      601 ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaatttc gtatggcaat
      661 gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga
      721 gcaaactgaa acgttttcat cgctctggag tgaataccac gacga
//
