LOCUS       sequence-108499-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet28a_RBPMS_FL
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(679..711)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(715..732)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(742..759)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             778..800
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    815..839
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(839..858)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(840..858)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgctca
       61 gcagaactga cgggacttcc agccctgaga agtagcctcg gagggaggga gccagcgcat
      121 ctgggcatgc agtgaagcgg gataggtgaa agcaggagga ggtagagcag gcgctaactc
      181 cgctgggtac agagggtacg gggcccacac ttcagggcta ctggggtaaa gtgcaggcac
      241 tgtgagctca tatggctctc tggcaatgaa ctgaggtaca gtgttgggca gaggagtact
      301 ggggtttgga gtccctacga gtttgttctt ggccatcttc gtgtttgcct tagcaaactc
      361 tagtcgtagt gtttgcggaa tttcaggatc gaagcggatg ccattcaaag cattctttgc
      421 agcctctgct tctgagcgac tgtcaaaact gacaaaacct acaggctgtt tagatgtgag
      481 ctttataaga gaaccctcat agcccttaaa tggtctgaaa agcagataga gctcccgagg
      541 tttgatatcc agaggaaggc cactgacaaa tagggtccgg acctcctcct cctgaaggtt
      601 ggcctcgctc ggggtgttct ccttctcggc tttgccgccg ttgttcatgg gtcgacggag
      661 ctcgaattcg gatccgcgac ccatttgctg tccaccagtc atgctagcca tatggctgcc
      721 gcgcggcacc aggccgctgc tgtgatgatg atgatgatgg ctgctgccca tggtatatct
      781 ccttcttaaa gttaaacaaa attatttcta gaggggaatt gttatccgct cacaattccc
      841 ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atcctcta
//
