LOCUS       sequence-108501-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO-H2B-6
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      3..12
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(25..402)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     promoter        complement(491..694)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(520..540)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 caccatggtg gcgaccggtg gatccttagc gctggtgtac ttggtgatgg ccttagtacc
       61 ctcggacacg gcgtgcttgg ccaactcccc aggcagcagc aggcgcacgg ccgtctggat
      121 ctccctggag gtgatggtcg agcgcttgtt gtaatgcgcc aggcgggaag cctcacctgc
      181 gatgcgctcg aaaatgtcgt tcacaaacga attcatgatg cccatggcct tggacgaaat
      241 gccggtgtca gggtggacct gcttcagaac cttgtacaca tagatggaat agctctcctt
      301 gcggctgcgc ttgcgcttct tgccgccttt cttctgcgcc ttagtcaccg ccttcttgga
      361 gccctttttc ggggcgggag cagacttcgc tggctctggc atggtggcgg taccgtcgac
      421 tgcagaattc gaagcttgag ctcgagatct gagtccggta gcgctagcgg atctgacggt
      481 tcactaaacc agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
      541 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
      601 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
      661 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt
      721 agatgtactg ccaagtagga aagtcccata aggtcatgta ctggg
//
