LOCUS       sequence-108514-         767 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A member of an RBS library for mRFP1 with different translation 
            rates.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 193 to 227)
  AUTHORS   Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM
  TITLE     Efficient search, mapping, and optimization of multi-protein genetic
            systems in diverse bacteria.
  JOURNAL   Mol Syst Biol. 2014 Jun 21;10:731. doi: 10.15252/msb.20134955.
  PUBMED    24952589
REFERENCE   2  (bases 1 to 767)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-06-21"; volume: "10:731. doi"; pages: " 
            10.15252/msb.20134955"
COMMENT     Sequence Label: mRFP1-31
FEATURES             Location/Qualifiers
     source          1..767
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(193..227)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     promoter        280..382
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(449..468)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tttggtaact ttcagtttag cggtctgggt accttcgtac ggacgacctt caccttcacc
       61 ttcgatttcg aactcgtgac cgttaacgga accttccata cgaactttga aacgcatgaa
      121 ctctttgata acgtcttcag agctcgccat gaataccccc tgaagttgtg atcgtcgacg
      181 tcgtttctag aagctagcac tgtacctagg actgagctag ccgtcaacgg atcccctagg
      241 gaattccgcg ctcgaggata tctggcgaaa atgagacgtt gatcggcacg taagaggttc
      301 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      361 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      421 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      481 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      541 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      601 tcgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      661 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      721 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtg
//
