LOCUS       sequence-108527-         690 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a NADPH dependent blue fluorescent protein expressed under a
            strong promoter and strong RBS without His-tagged. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Ng CY, Farasat I, Maranas CD, Salis HM
  TITLE     Rational design of a synthetic Entner-Doudoroff pathway for improved
            and controllable NADPH regeneration.
  JOURNAL   Metab Eng. 2015 May;29:86-96. doi: 10.1016/j.ymben.2015.03.001. Epub
            2015 Mar 10.
  PUBMED    25769287
REFERENCE   2  (bases 1 to 690)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-05"; volume: "29:86-96. doi"; pages: " 
            10.1016/j.ymben.2015.03.001. Epub 2015 Mar 10"
COMMENT     Sequence Label: pCN-mBFP
FEATURES             Location/Qualifiers
     source          1..690
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     239..257
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcatgcgaaa cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga
       61 tccttggcgg caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg
      121 gcgccccagc tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc
      181 gggtaccgtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
      241 taggcgtatc acgaggccct ttcgtcttca cctgtcgact tcttgagcac agctaacacc
      301 acgtcgtccc tatctgctgc cctaggtcta tgagtggttg ctggataacg acgtcttttt
      361 tttgacagct agctcagtcc taggtataat atattcaggg agaccacagg atccacggtt
      421 tccctctaca aataattttg tttaactttt actagaggaa ttcttaaaga cccaggacga
      481 tttaaggagg agaaaagaca tgcagaatct gaacggcaaa gtggctttcg tgaccggcgg
      541 cagccgcggc atcggcgcgg cgatcgtccg ccgcttggcg gcggacggcg ccgacatcgc
      601 gttcacctat gtcagcgcct cgtcgaaaaa cgtggccacc gccctggtgc aagaactcga
      661 ggccaagggc cgccgcgctc gcgccatcca
//
