LOCUS       sequence-108528-         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes a NADPH dependent blue fluorescent protein expressed under a
            strong promoter and strong RBS without His-tagged. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Ng CY, Farasat I, Maranas CD, Salis HM
  TITLE     Rational design of a synthetic Entner-Doudoroff pathway for improved
            and controllable NADPH regeneration.
  JOURNAL   Metab Eng. 2015 May;29:86-96. doi: 10.1016/j.ymben.2015.03.001. Epub
            2015 Mar 10.
  PUBMED    25769287
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2015-05"; volume: "29:86-96. doi"; pages: " 
            10.1016/j.ymben.2015.03.001. Epub 2015 Mar 10"
COMMENT     Sequence Label: pCN-mBFP
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(20..114)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 tctcgccaag ctagcttgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc
       61 tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaagct
      121 cagctaatta agctttcaag cggcgaagcc gccgtccacc gccagactcg caccggtcac
      181 gtagcgccca tccggcccgg ccaggaaagc caccatgccg gcgatgtcgc gcacctcgcc
      241 gtaatgaggc aaggacagca cggccaccag ttcgcccgag cgttcgccat ctgcgggatt
      301 catgtcggtg tcgatcgggc ccgggtgcac gacgttggcg gtgatgccgc gcgcgcccag
      361 gtcgcgcgcc aggccgcgcg tcatgcccag cagcgccgac ttgctggcgg catacagcgt
      421 taccccggct cggccggcgc gttccgccag gcagctgccg atgttgatga tgcggccgcc
      481 gtccggcatc gaggcctgcg cggcctggat ggccacgaaa ggcgcgcgca cattgatgtt
      541 catcgtgcgt tcgtagtcgt ccagcgtcac ctcgcccaag gggccggcca ggaagatgcc
      601 ggcgttgttc accagcacgt ccaccggccc cagttgcacg atggcctgct cgaccgcctg
      661 ccgcacctgg gccggatccg ccgagtccgc ctggatggcg cgagcgcg
//
