LOCUS       sequence-108532-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Red fluorescent calcium sensor protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Dana H, Mohar B, Sun Y, Narayan S, Gordus A, Hasseman JP, Tsegaye G,
            Holt GT, Hu A, Walpita D, Patel R, Macklin JJ, Bargmann CI, Ahrens 
            MB, Schreiter ER, Jayaraman V, Looger LL, Svoboda K, Kim DS
  TITLE     Sensitive red protein calcium indicators for imaging neural 
            activity.
  JOURNAL   eLife. 2016 Mar 24;5. pii: e12727. doi: 10.7554/eLife.12727.
  PUBMED    27011354
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "eLife."; 
            date: "2016-03-24"; volume: "5. pii: e12727. doi"; pages: " 
            10.7554/eLife.12727"
COMMENT     Sequence Label: pGP-CMV-NES-jRCaMP1b
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             129..146
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             150..182
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(186..194,195..209)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggactca gatctcgcca
       61 ccatgctgca gaacgagctt gctcttaagt tggctggact tgatattaac aagactggag
      121 gaggttctca tcatcatcat catcatggta tggctagcat gactggtgga cagcaaatgg
      181 gtcgggatct gtacgacgat gacgataagg atctcgcaac aatggtcgac tcatcgcgac
      241 gtaagtggaa taagtggggt cacgcagtca gagctatagg tcggctgagc tcagcgaaca
      301 acaccgaaat gatgtaccca gcggatggtg gtctgcgtgg ttacactcac atggcgctga
      361 aagttgatgg cggcggtcac ctgtcctgtt ctttcgtgac cacctaccgc tccaaaaaga
      421 ctgtcggcaa cattaagatg cctgccattc attacgtcag ccaccgtctg gagcgcctgg
      481 aggagagcga taacgaaatg tttgtcgtac agcgtgaaca cgcagttgcc aagtttgtgg
      541 gcctgggtgg tggcggcggt accggaggga gcatgaactc cctgatcaag gagaacatgc
      601 gtatgaaagt ggttctggaa ggctccgtaa acggccacca gttcaaatgc actggtgaag
      661 gcgaaggcaa cccgtatatg ggcacccaga ctatgcgtat caaagtgatc gagggtggtc
      721 cgctgccgtt tgcgttcgac atcctggcga cgtcctttat gtatggctcc cgtaccttca
      781 tcaaatatcc gaaaggcatc ccggatttct ttaagcagtc cttcccggaa ggttttacct
      841 ggg
//
