LOCUS       sequence-108553-        8895 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Episomal E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG227
FEATURES             Location/Qualifiers
     source          1..8895
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      7..1349
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(970..1017)
                     /label=FRT (mutant)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT site with a mutation in the core sequence (Azam 
                     et al., 1997)"
     protein_bind    complement(970..1017)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(1664..5842)
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     promoter        complement(5843..6082)
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     primer_bind     6184..6206
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6198..6215
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6199..6215
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6222..6241
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6222..6240
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     6349..6365
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6349..6365
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(6399..6415)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6400..6416)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(6514..6532)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6553..6569)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6553..6569)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6566..6588)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6577..6593
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(6967..6984)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7138..7726)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7218..7237)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7905..8696,8697..8765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8528..8547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8766..8870)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc gagagcgcta
       61 atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa cgcgaaagcg
      121 ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc aacgcgagag
      181 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
      241 gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa aatgcatccc
      301 gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt tgtgcgctct
      361 ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag aagaaggcta
      421 ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg cgtttactga
      481 ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat tatattctat
      541 accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg attcttcatt
      601 ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt ataggaaatg
      661 tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac aatttttttg
      721 tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt agatgcaagt
      781 tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag atatatagca
      841 aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta gtagctcgtt
      901 acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt tggttttcaa
      961 aagcgctctg aagttcctat actttttaga gaataggaac ttcggaatag gaacttcaaa
     1021 gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact
     1081 gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc
     1141 atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg
     1201 tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca
     1261 gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc
     1321 aatgctatca tttcctttga tattggatca tattaattaa ttggcgcgcc tgtgaagatc
     1381 acaaggtttt gcattgagga tagtatagaa gcaagaatca ttgaattaca ggaaaaaaag
     1441 gcaaatatga ttcatgctac aataaaccaa gatgaagctg ccattagcag actaacgcca
     1501 gccgatttac agttcttatt caataactaa tattttattc tcttattata tattattctc
     1561 ggagttttta agtgacatca cccgaaaaga agctaagtct ttctcctaat tcatatttaa
     1621 ttattgtaca tggacatatg catatcatac gtaatgctca accttaagct gctgcggagc
     1681 ttccacgagc accagcacct gaagcaacta gacttatttg cgcttgagtt agttctatac
     1741 ttggcagtgg aagtttgtca ttatgagttg gtggaggtaa aaatccaacc ttgtttaaaa
     1801 atgcaatata tataccaacc tcttctggag taacacctat tccattagac caatcaacac
     1861 ctgtccatgc ggtgtctttc tttaaagatg ccacggcgtt cctatcgtct agttccggag
     1921 ctttggtact ttcaggtaag ttgtctaaga ccatgtgtag caaaggatac aacgcatttt
     1981 cttcattcct gtcaataaca gacgcctcca atgatttctt ccatttagaa tagctttcga
     2041 tttcgacatc gtaaccataa tcgtgtaaag tatacaagta gtctttgaat aatattcttg
     2101 ggtgacccgt tacttgagca acggccaatt cattttcttt gggaggattc aaagacgtag
     2161 caacaacaac acgagcaaca tgatctactg gaaccatatt cacggaattt tcgatgtctg
     2221 gaatcttacc taattggact gaacctttca aaaatctcaa taagaaatca tctgtgtttg
     2281 aagaaccatt ggcagaggca cctgttacgt aacctggtct gacaatacac ccacgtaggc
     2341 ccctttcacc tgcacgtcta atgatgtact cagcagccca tttggactga ccatatccac
     2401 cagtgagccc gcttgcagag ttcattaaat cgtctgattc taaaatgcct ggcttccctt
     2461 cgctaacaag tttatctgac aaattaaagt agtattcagt gtcaagagta gaagtggagg
     2521 aaacaaagtc aaagaacttt ggcttgccga cggcggctaa gctcataaca ttgatagttg
     2581 aaataacatt tggatctctc aatttggcgt atggataaac ccagtgaact aacgcaccat
     2641 tatggataat tatatcaact gtgtttgcca aatccatcca cttctcatct gaaagaccaa
     2701 attggctttt agataaatcg cctaatacaa ctttaatatt tgaggcaaat ttttcgttcc
     2761 aagtaccata ggtgatacct gccttttgta atcttgcaaa tgcagcttct tcatccttgg
     2821 ccctgacgtg ggcaaacact ttgaaactgt agttctttgg agaacgtcct aacaaatctg
     2881 caaggatgta ggagcccaga aatcctgtga caccagtaac aaacacatta attgttgttt
     2941 ttccttcggc actattaggt tcaacaaaat attcacgaga ggggtacgaa cttggtagcg
     3001 tctcaaccaa tttcttggcg tcttccgcat aatttgcagt gacattttcg acgacctcac
     3061 cttgagatga tccacccgat gatttaattc tgtcaatttc cgcggcaaag gcctttatcg
     3121 ttggatactt gaaaattgtg cccaatggta aatcaacttg cagctttttc tttaaggtaa
     3181 aaatcatttt ggtagccaag atagaatgac cacctaaatc gaaaaacgaa tcatctggtg
     3241 atacagatgc tggcttggta ggtaatatac ttaaccataa gtctctaacc tcgcgctcaa
     3301 cattggtaaa ctgagagtcg tcagtttcag aaactgtatt ttcagctacc aaatttaatt
     3361 gcttgggagt tgggaattga agtttaggct tatcaacttt accatttgga ttcaatggta
     3421 gtttatccat aaccacaatc aaggaaggca tagcatagct agccaatctt ttctttaaga
     3481 aagtcctgat gtccttggat aaaagatggt aaccgattaa gcccttaact atagggtcag
     3541 tttcaacctc ctttggaaca tcactttgga acttagacaa gtcatctggc ttgtcaaatc
     3601 ttgggaccat aaatgtgatc aatgttggct cattgtcggc atttttgcga actaaagtaa
     3661 tgttttctct taccaatgga tgttgggaaa tgtgcgtatc tatttctcct aattcgattc
     3721 tgaacccacg aattttaact tgatcatcag ccctaccgca acattcacag tcaccgtttg
     3781 gtagataacg acctaaatca cccgttctgt acaatctatc tcttggacct aaccagaatt
     3841 gtctccaagg ttcaccatta tccttatcca aataattcca gtgatctttt tcaacaaacc
     3901 agttgttcac aaatttttct ttattcaatt ctggtaatcc tctataacct tcggccaaac
     3961 cacctgcacg aacataaatc tcacctattt cgccaatacc acatatttga gtacggtcgt
     4021 tcctgttaac aactaatagc tgaacgttca acataccttt accagcaggc atgacatctt
     4081 tcaatttttt caaaaagttt ggatcgtcat tttttgattt aacttcgaaa taagaaactg
     4141 cacgctgtgt ttcagtagta ccgtacatat taacaatacg gcaattttct gccaaggttt
     4201 gtaacctcag acaatcacgt tttgttaaaa tgtcacccac aaagaacgca tgatgtaact
     4261 tagggaatgg tgtagtagct tgggcagtaa gtaattgacc catggcaggt gttaaatggg
     4321 taactgtgca accatactta ctcatccatt ccgctaaacg gcccggtgta ccaatatcat
     4381 cttgagtagg gacatacaat tgggcaccta aaaataatgg tgtaaacata tctctttgaa
     4441 ttggatcatg tgcaataccg ctcagcattg tgaatttatc attttctgtt aagttgaacc
     4501 ttttggacat ccaattgaaa taataagcca aggaaaaatg tctaccaaga acacccttag
     4561 gaataccttc ggaaccagat gtgaaagata gggttgggtt ggaatctggt ccaactacaa
     4621 cacctgttct ggtgtctttg tagtgatcgt atggagccaa aacatcctcg ccattgtcca
     4681 atttgccacc ttcaatagta ccattttctt gaatagcgat ggaattgatt cttgaaacaa
     4741 tctccaattc atcattgatg taatcttcta ctaattgatc caattgtcca gcagctctaa
     4801 taacaatcaa cccacgtggt ttagcaacac ctaagtaaat ggtttgtctg gctgggggat
     4861 atgcagggtc gataactgaa aaggttgcgc cagctttcaa gacacccatc acacatacca
     4921 tcaaatccac acccctagaa gaatagatca tcactacatc acctcttttg atacctgttt
     4981 taatcaaata atgggcaact atgttagaag tgcggttgat gtcgcgataa gtgaaagaac
     5041 gggacttgtc ggaatttagt gttggagtct ccacaacaca ggttctctct gggaaggctt
     5101 cagcattgtc ctggaaaatg tcgtgaatac accccacgaa atcgcaccag cccaagttct
     5161 tagttggatc aggtaaacta tccttggatg atgcggtgat cagagagatt ttagttatgc
     5221 aattggatgg atcgcttagc gcagcagtca aatattgagt aaattggtcc gcaacaatgg
     5281 ttactctttc attcgaatac agtaagctgt tataaattaa gttcaaaaca tgcgcattat
     5341 tactggtatc caaattcaaa gcaaagtcca ctaaatgatg cttgaactcg tctaatttga
     5401 aatcttggtt ttccaaaaag gccaaacgga acaactgagg ggtcctttcc aagtcttggc
     5461 aactttgaat tttttcagct agctcgtcaa aggaaaaatt ggcctcaata gaattgagct
     5521 tgttcaactc attgttaatt gtagaataca gctcattaaa tgaccacgtt ggttgaatat
     5581 tgaatcttaa gattttgtta ttcgcaatat aaagaacaat atcatcgtca ccagttactc
     5641 tatatatcaa tgcagcccat acactcaaag cgacagcgta tttgttagaa aaactatcat
     5701 gaggcacatc gagctgaggt agctgtaacg aatatgtagc ttgtttcgta taaggttctt
     5761 gttgtgggcg taaaaagtca tgtggtaaca ctgaaagagt tggattatcc aacttctcta
     5821 tccagacctt ttcgttagtc attagtgagt aactctgtga tatgtctcta taattagcag
     5881 tttttcactg aaattccagg aaaggtaata aactcagatt tttttttata ctattggctg
     5941 cttgttactt atatatcttg aacttctccc agcgggtctt caaatacatt tgggcgatgt
     6001 tcatgttcat taggcaggta tttcgacatt gagtcacacg cgaaaaaccg ccggaatttt
     6061 ttatgtaatt gcaagtgcaa ttccgctggc aaaactattg aagagttttc ctcgctaaaa
     6121 ctgtgcgatg cctctcgatc ggatgtgctg caaggcgatt aagttgggta acgccagggt
     6181 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
     6241 gcgaattgga gaaacagaat aatatataaa cctgtataat ataaccttga agactatatt
     6301 tcttttcgcg gccgcctata gggcgaattg gagctccacc gcggtggctc tagaactagt
     6361 ggatcccccg ggctgcagga attcgatatc aagcttatcg ataccgtcga cctcgagggg
     6421 gggcccggta cccagcttcg gccgtattta cagataacga tgtcgttatt atatatatat
     6481 atatatattg tcacttgtac ccagcttttg ttccctttag tgagggttaa tttcgagctt
     6541 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caacagctga
     6601 gaagcggtca gcaagaagaa aatcgaataa tcagtttcaa tgaatataga cgtaacaaag
     6661 gcacaaaaaa gaaaacaagt agatatgtca tacatatata tatatttcag gcagcaagtg
     6721 accatcaacc aggaagtgtg aaatagcaaa gaactgttta cttcttcaga cttagaaagc
     6781 tcttcccata ggagaaagca actgctttcc tttgtagtaa ttgattgtct tttcagccct
     6841 tgacgcaaca ccgtccgctg tgaaatcgaa caatttgtaa atttcaggcg cgccatcctg
     6901 caggcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     6961 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     7021 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     7081 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     7141 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     7201 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     7261 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     7321 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     7381 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     7441 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     7501 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     7561 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     7621 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     7681 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     7741 cttttgttta aacctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7801 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7861 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7921 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     7981 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8041 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8101 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8161 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8221 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8281 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8341 cctccgatgg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8401 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8461 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8521 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8581 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8641 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8701 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8761 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8821 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8881 cgaaaagtgc cacct
//
