LOCUS       sequence-108556-        5193 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrative E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG205MX
FEATURES             Location/Qualifiers
     source          1..5193
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2597..2619
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2611..2628
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2612..2628
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2635..2654
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2635..2653
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2762..2778
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2762..2778
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2812..2828)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2813..2829)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(2927..2945)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2966..2982)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2966..2982)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2979..3001)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2990..3006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(3265..3282)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3436..4024)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3516..3535)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4203..4994,4995..5063))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4826..4845
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5064..5168)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgaaa gaacgcggca ttagcaacat ttgcgaagcg aacgattttg cgaactgcta
       61 ttaattaact ctggcgcgcc taactttttg tgtggtgccc tcctccttgt caatattaat
      121 gttaaagtgc aattcttttt ccttatcacg ttgagccatt agtatcaatt tgcttacctg
      181 tattccttta catcctcctt tttctccttc ttgataaatg tatgtagatt gcgtatatag
      241 tttcgtctac cctatgaaca tattccattt tgtaatttcg tgtcgcatat gatccgcagg
      301 ctaaccggaa cctgtattat ttagtttatg ctacgttaaa taaagacctt tcgttcacat
      361 aactgaatgt gtaatggcct tgagatttca agcataccaa gttggtggag acggggtcgt
      421 tacaaaagac tctttaagcc aagatttcct tgacagcctt ggcgatagca tcgccaacct
      481 cagtggtaga gttagaacca ccaaggtcac cggttctgac acctgcatcc aagacatttc
      541 taacagcttc ttcaagagcc ctaccttctt caaccaaatc caaggataac ttcaacatca
      601 tagctgcaga taagatggta gcaattgggt taaccttgtt tgctggtaaa tctggggcag
      661 aaccatgaca tggttcgtac aaaccgaatg ccttgttagt gtcaggtagg gaagctagag
      721 atgcagaagg taataaaccc aaagaacctg gaataacaga ggcttcatcg gagataatat
      781 caccaaacat gttgttggta ataacaacac cgtttagctt agttggtgat ttaaccaaaa
      841 tcatagcagc agagtcgatc aattggtgct gaacagttaa ttgtgggaac tcagtcttga
      901 tggtttcttc aacagtcttt ctccacaatc tggaagaggc aagcacgtta gccttgtcaa
      961 gagaccagat tggtaatggt gggttttgtt gcaatgccaa gaaagcagcc attcttgtaa
     1021 ttctttgaac ttcaggaaca ctgtatttct cagagtccca agcaactccg tcaccttcat
     1081 cttcttttct ttcaccaaag tagataccac caaccaattc tctaacgacg acgaaatcag
     1141 taccctttgc atattcaggc ttcaaaggag aaagatctag taaagaatca gaagcaaagt
     1201 tacatggtct caagttggcg tatagaccca attcctttct gatcttcaat agaccttgtt
     1261 ctggtctaac tgcgcccgta ccccattttg gaccaccaac agcacctagt aagacagcat
     1321 cggctttctt agaggcttca agagcttcat ctggtaaagg agtgccagtg gcatcgatgg
     1381 cagcaccccc gatcaagtga tgttggaaat tgaacttaat ttctggacgg acttcagcaa
     1441 tggcattcaa gaccttaata gcttcgtcag taacttcttt accgacgtga tcacccggta
     1501 ggacaacgat attcttagac atggtataat ctgtgtagtg tgggatactt tttacttctt
     1561 caaataggta tcaacaataa aaataaatcg aagcaaatgt aggaatgcgt taaagcagat
     1621 gtacttactt agagtacata gatatattta tataattcaa tatataaaag tatatgaagc
     1681 atctgatgtt gaacctgtca tgactctaaa ctggaatggg tagttatgct tctgaaggtt
     1741 ttccgttaga aaatgagtgc agggatcagg cccggaaccg gttttagcct gaaaggaaaa
     1801 gaaaaaaatg cggtgattac tagcacgtga ctgcgctgaa ttggaatcta ccacaagtac
     1861 agcgaatggc aagagggaag gggaatatat tgtgcctggc ggtagcgtct gctatatcaa
     1921 atgttaaaca taactaaatg cagtagtgaa tagagtaatg tatttctaca tctgtgggta
     1981 gggccaatgg gagcccgatg tctattgtac agatattttc cttttatagt taatcaattc
     2041 aacttggaaa tcattgagtc gatgtctact tggataactg gagccgtggc agcgtttgaa
     2101 atttgacgag tcttcttgtt ttgttcgcgc attttcagtt tattcaaacg ggagatggca
     2161 ccgaatccac tgacttctct gttcaatttg aatggtaata ggaagaaagt acatttcgag
     2221 aaattttcgc ccaacgtcga aatctcatgg atgatttcct cgatacaaat gacaccttca
     2281 tcacctaatt tctcttcaac gatattgttg tcattcaaga ttatctcttt tggttcttta
     2341 tcttctggcc tttgccacat tattctagtt ctcttttgga ttaaagaacg gatagatgca
     2401 agtgaaggtt gtccgataac aatatatggt gatacaagtt tcaacaatgg gtacacgtcc
     2461 ttggtaagtt tgatgaaaac accagtgttc aactcaacaa gtctcaaaag agataacact
     2521 ttgtaagcct ttgctgggat cttcacagcg atcggatgtg ctgcaaggcg attaagttgg
     2581 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac
     2641 gactcactat agggcgaatt ggagaaacag aataatatat aaacctgtat aatataacct
     2701 tgaagactat atttcttttc gcggccgcct atagggcgaa ttggagctcc accgcggtgg
     2761 ctctagaact agtggatccc ccgggctgca ggaattcgat atcaagctta tcgataccgt
     2821 cgacctcgag ggggggcccg gtacccagct tcggccgtat ttacagataa cgatgtcgtt
     2881 attatatata tatatatata ttgtcacttg tacccagctt ttgttccctt tagtgagggt
     2941 taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3001 tcacaacagc tgtaatatat agtagtaacc tgaaaaatag tcaatgcccg tggttcattt
     3061 gccaatagaa cagtcgatgt gatcaatgta cacgaaagtc aatataaaat atccaaatag
     3121 ccaatggtgt caggacggct aaggcaaatg caactttgaa atacttattc ggtttgcatc
     3181 ttggcgcgcc tcttcctgca ggcattaatg aatcggccaa cgcgcgggga gaggcggttt
     3241 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     3301 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     3361 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     3421 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     3481 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     3541 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     3601 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     3661 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     3721 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     3781 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     3841 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     3901 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     3961 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4021 tcaagaagat cctttgatct tttgtttaaa cctacggggt ctgacgctca gtggaacgaa
     4081 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4141 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4201 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     4261 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     4321 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     4381 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     4441 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     4501 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     4561 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4621 gcggttagct ccttcggtcc tccgatggtt gtcagaagta agttggccgc agtgttatca
     4681 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4741 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4801 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     4861 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4921 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4981 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     5041 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     5101 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     5161 gttccgcgca catttccccg aaaagtgcca cct
//
