LOCUS       sequence-108557-        4434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrative E. coli/S. cerevisiae shuttle vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Gnugge R, Liphardt T, Rudolf F
  TITLE     A shuttle vector series for precise genetic engineering of 
            Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2015 Dec 9. doi: 10.1002/yea.3144.
  PUBMED    26647923
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2015
            Dec 9. doi: 10.1002/yea.3144."
COMMENT     Sequence Label: pRG206MX
FEATURES             Location/Qualifiers
     source          1..4434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(340..537)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     promoter        complement(1356..1699)
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     primer_bind     1787..1809
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1801..1818
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1802..1818
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1825..1844
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1825..1843
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1952..1968
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1952..1968
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2002..2018)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2003..2019)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(2117..2135)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2156..2172)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2156..2172)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2169..2191)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2180..2196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(2506..2523)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2677..3265)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2757..2776)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3444..4235,4236..4304))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4067..4086
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4305..4409)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacgtcgaaa gaacgcggca ttagcaacat ttgcgaagcg aacgattttg cgaactgcta
       61 ttaattaact ctggcgcgcc actacttttt ggaactcttg ttgttctttg gagttcaatg
      121 cgtccatctt tacagtcctg tcttattgtt cttgatttgt gccccgtaaa atactgttac
      181 ttggttctgg cgaggtattg gatagttcct ttttataaag gccatgaagg tttttctttc
      241 caattttttt tttttcgtca ttatagaaat cattacgacc gagattcccg gcatatgcgt
      301 tttcgacact ggatggcggc gttagtatcg aatcgacagc agtatagcga ccagcattca
      361 catacgattg acgcatgata ttactttctg cgcacttaac ttcgcatctg ggcagatgat
      421 gtcgaggcga aaaaaaatat aaatcacgct aacatttgat taaaatagaa caactacaat
      481 ataaaaaaac tatacaaatg acaagttctt gaaaacaaga atctttttat tgtcagtact
      541 gattataatt ggccagtctt tttcaaataa gcattccaac cagcatctct atacctttta
      601 ccttcaatat ctggatctct tcctttacca aacaatcctc taccaacaat gataatatca
      661 gttccagtgc taacaacttc atcaacagtt ctatattgtt gtcctaatcc atcaccttta
      721 tcatctaatc caactccagg tgtcataata agccaatcaa atccttcttc ttgtccaccc
      781 atatcacgtt gggcaataaa tccaataaca aattccttat cggatttagc aatttcaaca
      841 gttttttgag aatattctcc ataagctaat gatcccactg atgataattc agctaacatc
      901 aataaccctc ttggctcttg gttggtggtg gtttctttag ctccctgttt taatccttca
      961 actactccat tcccagtgac accatgagca ttagtaatat ctgcccaact actaatttta
     1021 taaactccac caatatattg tttcttcacg gtattaccaa tatcagcaaa ttttctatct
     1081 tcaaaaatca taaattgatg tttacgtgaa agttctaata atggttcaat agtggattca
     1141 taggaaaaat cattgattat atcaatatga gtcttgatta agcatacata aggacccaat
     1201 ttatcaatta attcaagaaa ttccttagtg gtatcaacat caattgatgc acataaattg
     1261 gttttcttca gttccattaa tcgaaataat cgttgtgcta ctggtgaggc atgagtttct
     1321 gctctctcac tataggtctt agtgttgact gtcatggttg tttatgttcg gatgtgatgt
     1381 gagaactgta tcctagcaag attttaaaag gaagtatatg aaagaagaac ctcagtggca
     1441 aatcctaacc ttttatattt ctctacaggg gcgcggcgtg gggacaattc aacgcgtctg
     1501 tgaggggagc gtttccctgc tcgcaggtcc gcagcgagga gccgtaattt ttgcttcgcg
     1561 ccgtgcggcc atcaaaatgt atggatgcaa atgattatac atggggatgt atgggctaaa
     1621 tgtacgggcg acagtcacat catgcccctg agctgcgcac gtcaagactg tcaaggaggg
     1681 tattctgggc ctccatgtcg ctggccgggt gacccggcgg ggacgaggca agctaaaccg
     1741 atcggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     1801 tgtaaaacga cggccagtga attgtaatac gactcactat agggcgaatt ggagaaacag
     1861 aataatatat aaacctgtat aatataacct tgaagactat atttcttttc gcggccgcct
     1921 atagggcgaa ttggagctcc accgcggtgg ctctagaact agtggatccc ccgggctgca
     1981 ggaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg gtacccagct
     2041 tcggccgtat ttacagataa cgatgtcgtt attatatata tatatatata ttgtcacttg
     2101 tacccagctt ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat
     2161 agctgtttcc tgtgtgaaat tgttatccgc tcacaacagc tggatgaatt gaatagcttt
     2221 atggaccctg aaaccacagc cacattaacc ttctttgatg gtcaaaactt atccttcacc
     2281 ataaatatgc ctcgcaaaaa aggtaattaa catatataga attacattat ttatgaaata
     2341 tcatcactat ctcttagcat ctttaatcct tttctacatc agataacttc ggtttgttat
     2401 catcgtctgt attgtcatca attggcgcgc ctctccctgc aggcattaat gaatcggcca
     2461 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2521 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2581 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2641 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2701 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2761 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2821 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2881 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2941 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     3001 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     3061 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     3121 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3181 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3241 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttgtttaa acctacgggg
     3301 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     3361 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     3421 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     3481 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     3541 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     3601 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     3661 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     3721 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     3781 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     3841 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatggt tgtcagaagt
     3901 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     3961 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     4021 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     4081 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     4141 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     4201 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     4261 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     4321 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     4381 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acct
//
