LOCUS       sequence-108564-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway compatible BiFC vector (pEarleyGate202-YC).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller 
            WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y
  TITLE     Arabidopsis homolog of the yeast TREX-2 mRNA export complex: 
            components and anchoring nucleoporin.
  JOURNAL   Plant J. 2010 Jan;61(2):259-70. doi: 
            10.1111/j.1365-313X.2009.04048.x. Epub 2009 Oct 16.
  PUBMED    19843313
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-313X.2009.04048.x"; journalName: "Plant J"; date: 
            "2010-01"; volume: "61"; issue: "2"; pages: "259-70"
COMMENT     Sequence Label: pGTQL1221YC
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    30..84
                     /label=TMV Omega
                     /note="translational enhancer from the tobacco mosaic virus
                     5'-leader sequence (Gallie et al., 1988)"
     CDS             89..112
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    116..240
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(265..270,271..288,289..295)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(415..434)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgctcgagta taagagctct atttttacaa caattaccaa caacaacaaa caacaaacaa
       61 cattacaatt acatttacaa ttaccatgga ctacaaagac gatgacgaca aaatcacaag
      121 tttgtacaaa aaagctgaac gagaaacgta aaatgatata aatatcaata tattaaatta
      181 gattttgcat aaaaaacaga ctacataata ctgtaaaaca caacatatcc agtcactatg
      241 gcggccgcat taggcacccc aggctttaca ctttatgctt ccggctcgta taatgtgtgg
      301 attttgagtt aggatccgtc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
      361 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
      421 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
      481 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
      541 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
      601 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
      661 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
      721 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtt
//
