LOCUS       sequence-108590-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is designed to validate the biophysical model of 
            translational coupling.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Tian T, Salis HM
  TITLE     A predictive biophysical model of translational coupling to 
            coordinate and control protein expression in bacterial operons.
  JOURNAL   Nucleic Acids Res. 2015 Aug 18;43(14):7137-51. doi: 
            10.1093/nar/gkv635. Epub 2015 Jun 27.
  PUBMED    26117546
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gkv635"; journalName: "Nucleic Acids Res"; date: 
            "2015-08-18- 18"; volume: "43"; issue: "14"; pages: "7137-51"
COMMENT     Sequence Label: pTd-4-15-13-10
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             248..256
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 gagcgcatgt atccggagga cggcgcttta aaaggcgaaa tcaagatgcg cttaaagtta
       61 aaagacggcg gtcactacga cgcagaagtt aaaacgacgt acatggccaa gaaaccggtt
      121 cagttaccgg gcgcttacaa aaccgacatc aaactcgaca tcaccagcca caacgaagac
      181 tacaccatcg tcgaacagta cgaacgcgca gaaggtcgtc acagcaccgg tgctgaattc
      241 gtacacctaa ggaggtgtac agcatgaaac gtaaactcga ggaacttttc acgggggtcg
      301 tcccaattct ggtagaatta gacggtgatg taaacgggca caagtttagc gtcagcggag
      361 agggcgaggg ggacgccacc tacggtaaac ttacccttaa gtttatttgc acgacgggca
      421 aattacccgt tccgtggcca acgctcgtca ctactttcgg ctacggcgtt cagtgcttcg
      481 cgagatatcc ggatcacatg aagcagcacg atttcttcaa gtcggccatg ccagagggtt
      541 acgttcagga gagaactata tttttcaagg acgacggcaa ttacaagaca cgcgccgaag
      601 tcaagttcga aggcgatacc ctggttaacc gtatcgagtt aaaaggtatc gattttaaag
      661 aagacggaaa cattctcggt cacaaactcg aatacaacta taacagccat aatgtctaca
      721 ttatggctga caaacagaag aacggcatta aggttaactt caaaattcgc cacaacatcg
      781 aagacggtag cgtccaacta gcagaccatt atcaac
//
