LOCUS       sequence-108597-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-CD36-C-10
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             44..754
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVPKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcggtggcc accatggtgc ctaagggcga
       61 agagctgatt aaggagaaca tgcacatgaa gctgtacatg gagggcaccg tggacaacca
      121 tcacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
      181 aatcaaggtg gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctactagctt
      241 cctctacggc agcaagacct tcatcaacca cacccagggc atccccgact tcttcaagca
      301 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
      361 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaagatcag
      421 aggggtgaac ttcacatcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
      481 cttcaccgag acgctgtacc ccgctgacgg cggcctggaa ggcagaaacg acatggccct
      541 gaagctcgtg ggcgggagcc atctgatcgc aaacgccaag accacatata gatccaagaa
      601 acccgctaag aacctcaaga tgcctggcgt ctactatgtg gactacagac tggaaagaat
      661 caaggaggcc aacaacgaga cctacgtcga gcagcacgag gtggcagtgg ccagatactg
      721 cgacctccct agcaaactgg ggcacaagct taattccgga ctcagatctg gcagcagtgg
      781 aggtggctgt gaccggaact gtgggctcat cgctggggct gtcattggtg ctgtcctggc
      841 tgtgtttgga ggtattctaa tgccagttgg agacctgctt atccaga
//
