LOCUS       sequence-108598-        2252 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Membrane, Excitation: 399, Emission: 456.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Subach OM, Cranfill PJ, Davidson MW, Verkhusha VV
  TITLE     An enhanced monomeric blue fluorescent protein with the high 
            chemical stability of the chromophore.
  JOURNAL   PLoS One. 2011;6(12):e28674. Epub 2011 Dec 8.
  PUBMED    22174863
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(12)"; pages: "e28674. Epub 2011 Dec 8"
COMMENT     Sequence Label: mTagBFP2-CD36-C-10
FEATURES             Location/Qualifiers
     source          1..2252
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..739
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVPKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gacgtcagat ccgctagcgg tggccaccat ggtgcctaag ggcgaagagc tgattaagga
       61 gaacatgcac atgaagctgt acatggaggg caccgtggac aaccatcact tcaagtgcac
      121 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
      181 gggcggccct ctccccttcg ccttcgacat cctggctact agcttcctct acggcagcaa
      241 gaccttcatc aaccacaccc agggcatccc cgacttcttc aagcagtcct tccctgaggg
      301 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
      361 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttcac
      421 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccttca ccgagacgct
      481 gtaccccgct gacggcggcc tggaaggcag aaacgacatg gccctgaagc tcgtgggcgg
      541 gagccatctg atcgcaaacg ccaagaccac atatagatcc aagaaacccg ctaagaacct
      601 caagatgcct ggcgtctact atgtggacta cagactggaa agaatcaagg aggccaacaa
      661 cgagacctac gtcgagcagc acgaggtggc agtggccaga tactgcgacc tccctagcaa
      721 actggggcac aagcttaatt ccggactcag atctggcagc agtggaggtg gctgtgaccg
      781 gaactgtggg ctcatcgctg gggctgtcat tggtgctgtc ctggctgtgt ttggaggtat
      841 tctaatgcca gttggagacc tgcttatcca gaagacaatt aaaaagcaag ttgtcctcga
      901 agaaggtaca attgctttta aaaattgggt taaaacaggc acagaagttt acagacagtt
      961 ttggatcttt gatgtgcaaa atccacagga agtgatgatg aacagcagca acattcaagt
     1021 taagcaaaga ggtccttata cgtacagagt tcgttttcta gccaaggaaa atgtaaccca
     1081 ggacgctgag gacaacacag tctctttcct gcagcccaat ggtgccatct tcgaaccttc
     1141 actatcagtt ggaacagagg ctgacaactt cacagttctc aatctggctg tggcagctgc
     1201 atcccatatc tatcaaaatc aatttgttca aatgatcctc aattcactta ttaacaagtc
     1261 aaaatcttct atgttccaag tcagaacttt gagagaactg ttatggggct atagggatcc
     1321 atttttgagt ttggttccgt accctgttac taccacagtt ggtctgtttt atccttacaa
     1381 caatactgca gatggagttt ataaagtttt caatggaaaa gataacataa gtaaagttgc
     1441 cataatcgac acatataaag gtaaaaggaa tctgtcctat tgggaaagtc actgcgacat
     1501 gattaatggt acagatgcag cctcatttcc accttttgtt gagaaaagcc aggtattgca
     1561 gttcttttct tctgatattt gcaggtcaat ctatgctgta tttgaatccg acgttaatct
     1621 gaaaggaatc cctgtgtata gatttgttct tccatccaag gcctttgcct ctccagttga
     1681 aaacccagac aactattgtt tctgcacaga aaaaattatc tcaaaaaatt gtacatcata
     1741 tggtgtgcta gacatcagca aatgcaaaga agggagacct gtgtacattt cacttcctca
     1801 ttttctgtat gcaagtcctg atgtttcaga acctattgat ggattaaacc caaatgaaga
     1861 agaacatagg acatacttgg atattgaacc tataactgga ttcactttac aatttgcaaa
     1921 acggctgcag gtcaacctat tggtcaagcc atcagaaaaa attcaagtat taaagaatct
     1981 gaagaggaac tatattgtgc ctattctttg gcttaatgag actgggacca ttggtgatga
     2041 gaaggcaaac atgttcagaa gtcaagtaac tggaaaaata aacctccttg gcctgataga
     2101 aatgatctta ctcagtgttg gtgtggtgat gtttgttgct tttatgattt catattgtgc
     2161 atgcagatcg aaaacaataa aataagaatt ctgcagtcga cggtaccgcg ggcccgggat
     2221 ccaccggatc tagataactg atcataattc ag
//
