LOCUS       sequence-108663-        1043 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chr.13/CLYBL TALEN expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1043)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 1043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pZT-C13-L1
FEATURES             Location/Qualifiers
     source          1..1043
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             148..213
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             220..240
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gctgatccac
      121 tagtccagtg tggtggaatt cgccatggac tacaaagacc atgacggtga ttataaagat
      181 catgacatcg attacaagga tgacgatgac aagatggccc ccaagaagaa gaggaaggtg
      241 ggcatccacg gggtacctat ggtggacttg aggacactcg gttattcgca acagcaacag
      301 gagaaaatca agcctaaggt caggagcacc gtcgcgcaac accacgaggc gcttgtgggg
      361 catggcttca ctcatgcgca tattgtcgcg ctttcacagc accctgcggc gcttgggacg
      421 gtggctgtca aataccaaga tatgattgcg gccctgcccg aagccacgca cgaggcaatt
      481 gtaggggtcg gtaaacagtg gtcgggagcg cgagcacttg aggccttgct gactgtggcg
      541 ggtgagctta gggggcctcc gctccagctc gacaccgggc agctgctgaa gatcgcgaag
      601 agagggggag taacagcggt agaggcagtg cacgcctggc gcaatgcgct caccggggcc
      661 cccctgaacc tgaccccgga ccaagtggtg gctatcgcca gccacgatgg cggcaagcaa
      721 gcgctcgaaa cggtgcagcg gctgttgccg gtgctgtgcc aggaccatgg cctgaccccg
      781 gaccaagtgg tggctatcgc cagcaacggt ggcggcaagc aagcgctcga aacggtgcag
      841 cggctgttgc cggtgctgtg ccaggaccat ggcctgaccc cggaccaagt ggtggctatc
      901 gccagcaacg gtggcggcaa gcaagcgctc gaaacggtgc agcggctgtt gccggtgctg
      961 tgccaggacc atggcctgac cccggaccaa gtggtggcta tcgccagcaa cattggcggc
     1021 aagcaagcgc tcgaaacggt gca
//
