LOCUS       sequence-108674-        3321 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3321)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 3321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: myr-FLAG-PIK3CA-pcw107
FEATURES             Location/Qualifiers
     source          1..3321
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..42
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             67..90
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      3255..3264
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggggtctt caaaatctaa accaaaggac cccagccagc gccggcgcag gatccgaggt
       61 taccttgact acaaagacga tgacgacaag caattgagcg acgagatccg aacaatgcct
      121 ccaagaccat catcaggtga actgtggggc atccacttga tgcccccaag aatcctagta
      181 gaatgtttac taccaaatgg gatgatagtg actttagaat gcctccgtga ggctacgtta
      241 ataacgataa agcatgaact atttaaagaa gcaagaaaat accctctcca tcaacttctt
      301 caagatgaat cttcttacat tttcgtaagt gttacccaag aagcagaaag ggaagaattt
      361 tttgatgaaa caagacgact ttgtgacctt cggctttttc aacccttttt aaaagtaatt
      421 gaaccagtag gcaaccgtga agaaaagatc ctcaatcgag aaattggttt tgctatcggc
      481 atgccagtgt gtgaattcga tatggttaaa gatccagaag tacaggactt ccgaagaaat
      541 attctcaatg tttgtaaaga agctgtggat cttagggatc ttaattcacc tcatagtaga
      601 gcaatgtatg tttatcctcc aaatgtagaa tcttcaccag aactgccaaa gcacatatat
      661 aataaattgg ataaagggca aataatagtg gtgatttggg taatagtttc tccaaataat
      721 gacaaacaga agtatactct gaaaatcaac catgactgtg tgccagaaca agtaattgct
      781 gaagcaatca ggaaaaaaac tcgaagtatg ttgctatcat ctgaacaact aaaactctgt
      841 gttttagaat atcagggcaa gtatatttta aaagtgtgtg gatgtgatga atacttccta
      901 gaaaaatatc ctctgagtca gtataagtat ataagaagct gtataatgct tgggaggatg
      961 cccaatttga tgctgatggc taaagaaagc ctctattctc aactgccaat ggactgtttt
     1021 acaatgccat catattccag acgcatctcc acagctacgc catatatgaa tggagaaaca
     1081 tctacaaaat ccctttgggt tataaatagt gcactcagaa taaaaattct ttgtgcaacc
     1141 tatgtgaatg taaatattcg agacattgac aagatttatg ttcgaacagg tatctaccat
     1201 ggaggagaac ccttatgtga taatgtgaac actcaaagag taccttgttc caatcccagg
     1261 tggaatgaat ggctgaatta cgatatatac attcctgatc ttcctcgtgc tgctcgactt
     1321 tgcctttcca tttgttctgt taaaggccga aagggtgcta aagaggaaca ctgtccattg
     1381 gcctggggaa atataaactt gtttgattac acagatactc tagtatctgg aaaaatggct
     1441 ttgaatcttt ggccagtacc tcatggacta gaagatttgc tgaaccctat tggtgttact
     1501 ggatcaaatc caaataaaga aactccatgt ttagagttgg agtttgactg gttcagcagt
     1561 gtggtaaagt ttccagatat gtcagtgatt gaagagcatg ccaattggtc tgtatcccgt
     1621 gaagcaggat ttagttattc ccatgcagga ctgagtaaca gactagctag agacaatgaa
     1681 ttaagagaaa atgataaaga acagctccga gcaatttgta cacgagatcc tctatctgaa
     1741 atcactgagc aagagaaaga ttttctgtgg agccacagac actattgtgt aactatcccc
     1801 gaaattctac ccaaattgct tctgtctgtt aaatggaact ctagagatga agtagctcag
     1861 atgtactgct tggtaaaaga ttggcctcca atcaagcctg aacaggctat ggagcttctg
     1921 gactgcaatt acccagatcc tatggttcga ggttttgctg ttcggtgctt agaaaaatat
     1981 ttaacagatg acaaactttc tcagtaccta attcagctag tacaggtact aaaatatgaa
     2041 cagtatttgg ataacctgct tgtgagattt ttactcaaaa aagcgttaac taatcaaagg
     2101 atcggtcact ttttcttttg gcatttaaaa tctgagatgc acaataaaac agttagtcag
     2161 aggtttggcc tgcttttgga gtcctattgc cgtgcatgtg ggatgtatct gaagcacctt
     2221 aataggcaag ttgaggctat ggaaaagctc attaacttga ctgacattct caaacaagag
     2281 aagaaggatg aaacacaaaa ggtacagatg aagtttttag ttgagcaaat gcggcgacca
     2341 gatttcatgg atgctctcca gggctttctg tctcctctaa accctgctca tcagctggga
     2401 aatctcaggc ttgaagagtg tcgaattatg tcttctgcaa aaaggccact gtggttgaat
     2461 tgggagaacc cagacatcat gtcagaatta ctctttcaga acaatgagat catctttaaa
     2521 aatggggatg atttacggca agatatgcta acccttcaga ttattcgcat tatggaaaat
     2581 atctggcaaa atcaaggtct tgatcttcga atgttacctt atggatgtct gtcaatcggt
     2641 gactgtgtgg gacttatcga ggtggtgaga aattctcaca ctataatgca gattcagtgt
     2701 aaaggaggcc tgaaaggtgc actgcagttt aacagccaca cactccatca gtggctcaaa
     2761 gacaagaaca agggggaaat atatgatgcg gccatcgatt tgtttacacg atcatgtgct
     2821 ggatattgtg ttgccacctt cattttggga attggagatc gtcacaatag taatatcatg
     2881 gttaaagatg atggacaact gtttcatata gattttggac actttttgga tcacaagaag
     2941 aaaaaatttg gttataaacg agagcgcgtg ccgtttgttt tgacacaaga tttcttaata
     3001 gtgattagta aaggagccca agaatgcaca aagacaagag aatttgagag gtttcaggag
     3061 atgtgttaca aggcttatct agctattcgg cagcatgcca atctcttcat aaatcttttc
     3121 tcaatgatgc ttggctctgg aatgccagaa ctgcaatctt ttgatgatat tgcatacatt
     3181 cgaaagaccc tagctttaga taaaactgag caagaggctt tggagtattt catgaaacaa
     3241 atgaatgatg cacaccatgg tggctggaca acaaaaatgg attggatctt ccacacaatt
     3301 aagcagcatg ctttgaactg a
//
