LOCUS       sequence-108675-        1563 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: myr-FLAG-AKT1-pcw107
FEATURES             Location/Qualifiers
     source          1..1563
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..42
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             67..90
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    97..121
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             121..1560
                     /codon_start=1
                     /gene="AKT1"
                     /product="human serine/threonine protein kinase activated 
                     by growth factors"
                     /label=Akt1
                     /translation="MSDVAIVKEGWLHKRGEYIKTWRPRYFLLKNDGTFIGYKERPQDV
                     DQREAPLNNFSVAQCQLMKTERPRPNTFIIRCLQWTTVIERTFHVETPEEREEWTTAIQ
                     TVADGLKKQEEEEMDFRSGSPSDNSGAEEMEVSLAKPKHRVTMNEFEYLKLLGKGTFGK
                     VILVKEKATGRYYAMKILKKEVIVAKDEVAHTLTENRVLQNSRHPFLTALKYSFQTHDR
                     LCFVMEYANGGELFFHLSRERVFSEDRARFYGAEIVSALDYLHSEKNVVYRDLKLENLM
                     LDKDGHIKITDFGLCKEGIKDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMY
                     EMMCGRLPFYNQDHEKLFELILMEEIRFPRTLGPEAKSLLSGLLKKDPKQRLGGGSEDA
                     KEIMQHRFFAGIVWQHVYEKKLSPPFKPQVTSETDTRYFDEEFTAQMITITPPDQDDSM
                     ECVDSERRPHFPQFSYSASGTA"
ORIGIN
        1 atggggtctt caaaatctaa accaaaggac cccagccagc gccggcgcag gatccgaggt
       61 taccttgact acaaagacga tgacgacaag caattgacaa gtttgtacaa aaaagttggc
      121 atgagcgacg tggctattgt gaaggagggt tggctgcaca aacgagggga gtacatcaag
      181 acctggcggc cacgctactt cctcctcaag aatgatggca ccttcattgg ctacaaggag
      241 cggccgcagg atgtggacca acgtgaggct cccctcaaca acttctctgt ggcgcagtgc
      301 cagctgatga agacggagcg gccccggccc aacaccttca tcatccgctg cctgcagtgg
      361 accactgtca tcgaacgcac cttccatgtg gagactcctg aggagcggga ggagtggaca
      421 accgccatcc agactgtggc tgacggcctc aagaagcagg aggaggagga gatggacttc
      481 cggtcgggct cacccagtga caactcaggg gctgaagaga tggaggtgtc cctggccaag
      541 cccaagcacc gcgtgaccat gaacgagttt gagtacctga agctgctggg caagggcact
      601 ttcggcaagg tgatcctggt gaaggagaag gccacaggcc gctactacgc catgaagatc
      661 ctcaagaagg aagtcatcgt ggccaaggac gaggtggccc acacactcac cgagaaccgc
      721 gtcctgcaga actccaggca ccccttcctc acagccctga agtactcttt ccagacccac
      781 gaccgcctct gctttgtcat ggagtacgcc aacgggggcg agctgttctt ccacctgtcc
      841 cgggagcgtg tgttctccga ggaccgggcc cgcttctatg gcgctgagat tgtgtcagcc
      901 ctggactacc tgcactcgga gaagaacgtg gtgtaccggg acctcaagct ggagaacctc
      961 atgctggaca aggacgggca cattaagatc acagacttcg ggctgtgcaa ggaggggatc
     1021 aaggacggtg ccaccatgaa gaccttttgc ggcacacctg agtacctggc ccccgaggtg
     1081 ctggaggaca atgactacgg ccgtgcagtg gactggtggg ggctgggcgt ggtcatgtac
     1141 gagatgatgt gcggtcgcct gcccttctac aaccaggacc atgagaagct ttttgagctc
     1201 atcctcatgg aggagatccg cttcccgcgc acgcttggtc ccgaggccaa gtccttgctt
     1261 tcagggctgc tcaagaagga ccccaagcag aggcttggcg ggggctccga ggacgccaag
     1321 gagatcatgc agcatcgctt ctttgccggt atcgtgtggc agcacgtgta cgagaagaag
     1381 ctcagcccac ccttcaagcc ccaggtcacg tcggagactg acaccaggta ttttgatgag
     1441 gagttcacgg cccagatgat caccatcaca ccacctgacc aagatgacag catggagtgt
     1501 gtggacagcg agcgcaggcc ccacttcccc cagttctcct actcggccag cggcacggcc
     1561 tga
//
