LOCUS       sequence-108678-        2310 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2310)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 2310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: FLAG-IKKbeta (S177E,S181E)-pcw107
FEATURES             Location/Qualifiers
     source          1..2310
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atggactaca aagacgatga cgacaagctt gcggccgcaa tgagctggtc accttccctg
       61 acaacgcaga catgtggggc ctgggaaatg aaagagcgcc ttgggacagg gggatttgga
      121 aatgtcatcc gatggcacaa tcaggaaaca ggtgagcaga ttgccatcaa gcagtgccgg
      181 caggagctca gcccccggaa ccgagagcgg tggtgcctgg agatccagat catgagaagg
      241 ctgacccacc ccaatgtggt ggctgcccga gatgtccctg aggggatgca gaacttggcg
      301 cccaatgacc tgcccctgct ggccatggag tactgccaag gaggagatct ccggaagtac
      361 ctgaaccagt ttgagaactg ctgtggtctg cgggaaggtg ccatcctcac cttgctgagt
      421 gacattgcct ctgcgcttag ataccttcat gaaaacagaa tcatccatcg ggatctaaag
      481 ccagaaaaca tcgtcctgca gcaaggagaa cagaggttaa tacacaaaat tattgaccta
      541 ggatatgcca aggagctgga tcagggcgaa ctttgcacag aattcgtggg gaccctgcag
      601 tacctggccc cagagctact ggagcagcag aagtacacag tgaccgtcga ctactggagc
      661 ttcggcaccc tggcctttga gtgcatcacg ggcttccggc ccttcctccc caactggcag
      721 cccgtgcagt ggcattcaaa agtgcggcag aagagtgagg tggacattgt tgttagcgaa
      781 gacttgaatg gaacggtgaa gttttcaagc tctttaccct accccaataa tcttaacagt
      841 gtcctggctg agcgactgga gaagtggctg caactgatgc tgatgtggca cccccgacag
      901 aggggcacgg atcccacgta tgggcccaat ggctgcttca aggccctgga tgacatctta
      961 aacttaaagc tggttcatat cttgaacatg gtcacgggca ccatccacac ctaccctgtg
     1021 acagaggatg agagtctgca gagcttgaag gccagaatcc aacaggacac gggcatccca
     1081 gaggaggacc aggagctgct gcaggaagcg ggcctggcgt tgatccccga taagcctgcc
     1141 actcagtgta tttcagacgg caagttaaat gagggccaca cattggacat ggatcttgtt
     1201 tttctctttg acaacagtaa aatcacctat gagactcaga tctccccacg gccccaacct
     1261 gaaagtgtca gctgtatcct tcaagagccc aagaggaatc tcgccttctt ccagctgagg
     1321 aaggtgtggg gccaggtctg gcacagcatc cagaccctga aggaagattg caaccggctg
     1381 cagcagggac agcgagccgc catgatgaat ctcctccgaa acaacagctg cctctccaaa
     1441 atgaagaatt ccatggcttc catgtctcag cagctcaagg ccaagttgga tttcttcaaa
     1501 accagcatcc agattgacct ggagaagtac agcgagcaaa ccgagtttgg gatcacatca
     1561 gataaactgc tgctggcctg gagggaaatg gagcaggctg tggagctctg tgggcgggag
     1621 aacgaagtga aactcctggt agaacggatg atggctctgc agaccgacat tgtggactta
     1681 cagaggagcc ccatgggccg gaagcagggg ggaacgctgg acgacctaga ggagcaagca
     1741 agggagctgt acaggagact aagggaaaaa cctcgagacc agcgaactga gggtgacagt
     1801 caggaaatgg tacggctgct gcttcaggca attcagagct tcgagaagaa agtgcgagtg
     1861 atctatacgc agctcagtaa aactgtggtt tgcaagcaga aggcgctgga actgttgccc
     1921 aaggtggaag aggtggtgag cttaatgaat gaggatgaga agactgttgt ccggctgcag
     1981 gagaagcggc agaaggagct ctggaatctc ctgaagattg cttgtagcaa ggtccgtggt
     2041 cctgtcagtg gaagcccgga tagcatgaat gcctctcgac ttagccagcc tgggcagctg
     2101 atgtctcagc cctccacggc ctccaacagc ttacctgagc cagccaagaa gagtgaagaa
     2161 ctggtggctg aagcacataa cctctgcacc ctgctagaaa atgccataca ggacactgtg
     2221 agggaacaag accagagttt cacggcccta gactggagct ggttacagac ggaagaagaa
     2281 gagcacagct gcctggagca ggcctcatga
//
