LOCUS       sequence-108682-        2346 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: beta-catenin (S33A, S37A, T41A, S45A)-pcw107-V5
FEATURES             Location/Qualifiers
     source          1..2346
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             46..81
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
ORIGIN
        1 atggctactc aagctgacct gatggagttg gacatggcca tggagccgga cagaaaagct
       61 gctgtcagcc actggcagca gcagtcttac ttggatgctg gaatccatgc tggtgccacc
      121 gccacagctc ctgccctgag tggcaagggc aaccctgagg aagaagatgt tgacacctcc
      181 caagtccttt atgaatggga gcaaggcttt tcccagtcct tcacgcaaga gcaagtagct
      241 gatattgacg ggcagtatgc aatgactagg gctcagaggg tccgagctgc catgttccct
      301 gagacgctag atgagggcat gcagatccca tccacgcagt ttgacgctgc tcatcccact
      361 aatgtccagc gcttggctga accatcacag atgttgaaac atgcagttgt caatttgatt
      421 aactatcagg atgacgcgga acttgccaca cgtgcaattc ctgagctgac aaaactgcta
      481 aacgatgagg accaggtggt agttaataaa gctgctgtta tggtccatca gctttccaaa
      541 aaggaagctt ccagacatgc catcatgcgc tcccctcaga tggtgtctgc cattgtacgc
      601 accatgcaga atacaaatga tgtagagaca gctcgttgta ctgctgggac tctgcacaac
      661 ctttctcacc accgcgaggg cttgctggcc atctttaagt ctggtggcat cccagcgctg
      721 gtgaaaatgc ttgggtcacc agtggattct gtactgttct acgccatcac gacactgcat
      781 aatctcctgc tccatcagga aggagctaaa atggcagtgc gcctagctgg tggactgcag
      841 aaaatggttg ctttgctcaa caaaacaaac gtgaaattct tggctattac aacagactgc
      901 cttcagatct tagcttatgg caatcaagag agcaagctca tcattctggc cagtggtgga
      961 ccccaagcct tagtaaacat aatgaggacc tacacttatg agaagcttct gtggaccaca
     1021 agcagagtgc tgaaagtgct gtctgtctgc tctagcaaca agccggccat tgtagaagct
     1081 ggtgggatgc aggcactggg gcttcatctg acagacccaa gtcagcgact tgttcaaaac
     1141 tgtctttgga ctctcagaaa cctttcagat gcagcgacta agcaggaagg gatggaaggc
     1201 ctccttggga ctctagtgca gcttctgggt tccgatgata taaatgtggt cacctgtgca
     1261 gctggaattc tctctaacct cacttgcaat aattacaaaa acaagatgat ggtgtgccaa
     1321 gtgggtggca tagaggctct tgtacgcacc gtccttcgtg ctggtgacag ggaagacatc
     1381 actgagcctg ccatctgtgc tcttcgtcat ctgaccagcc ggcatcagga agccgagatg
     1441 gcccagaatg ccgttcgcct tcattatgga ctgcctgttg tggttaaact cctgcaccca
     1501 ccatcccact ggcctctgat aaaggcaact gttggattga ttcgaaacct tgccctttgc
     1561 ccagcaaatc atgcgccttt gcgggaacag ggtgctattc cacgactagt tcagctgctt
     1621 gtacgagcac atcaggacac ccaacggcgc acctccatgg gtggaacgca gcagcagttt
     1681 gtggagggcg tgcgcatgga ggagatagta gaagggtgta ctggagctct ccacatcctt
     1741 gctcgggacg ttcacaaccg gattgtaatc cgaggactca ataccattcc attgtttgtg
     1801 cagttgcttt attctcccat tgaaaatatc caaagagtag ctgcaggggt cctctgtgaa
     1861 cttgctcagg acaaggaggc tgcagaggcc attgaagctg agggagccac agctcccctg
     1921 acagagttac tccactccag gaatgaaggc gtggcaacat acgcagctgc tgtcctattc
     1981 cgaatgtctg aggacaagcc acaggattac aagaagcggc tttcagtcga gctgaccagt
     2041 tccctcttca ggacagagcc aatggcttgg aatgagactg cagatcttgg actggacatt
     2101 ggtgcccagg gagaagccct tggatatcgc caggatgatc ccagctaccg ttcttttcac
     2161 tctggtggat acggccagga tgccttgggg atggacccta tgatggagca tgagatgggt
     2221 ggccaccacc ctggtgctga ctatccagtt gatgggctgc ctgatctggg acacgcccag
     2281 gacctcatgg atgggctgcc cccaggtgat agcaatcagc tggcctggtt tgatactgac
     2341 ctgtaa
//
