LOCUS       sequence-108687-        2386 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mkk7-JNK2 fusion - barcoded activating construct of JNK pathway.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: FLAG-Mkk7-JNK2 fusion-pcw107
FEATURES             Location/Qualifiers
     source          1..2386
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(154..165)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 atggactaca aagacgatga tgacaaaatg gcggcgtcct ccctggagca gaagctgtcc
       61 cgcctggaag ccaagctgaa gcaggagaac cgtgaggccc gcaggaggat cgacctcaac
      121 ttggatatca gcccacagcg gcccaggccc accctgcaac tcccactggc caacgatggg
      181 ggcagccgct caccatcctc agagagctcc ccacagcacc ctacaccccc cacccggccc
      241 cgccacatgc tggggctccc atcaaccttg ttcacaccgc gcagtatgga gagcatcgag
      301 attgaccaga agctgcagga gatcatgaag cagacagggt acctgactat cgggggccag
      361 cgttatcagg cagaaatcaa tgacttggag aacttgggtg agatgggcag tggtacctgt
      421 ggtcaggtgt ggaagatgcg gttccggaag acaggccaca tcattgctgt taagcaaatg
      481 cggcgctctg ggaacaagga agagaataag cgcattttga tggacctgga tgtagtactc
      541 aagagccatg actgccctta catcgttcag tgctttggca ccttcatcac caacacagac
      601 gtctttattg ccatggagct catgggcaca tgtgcagaga agctgaagaa acgaatgcag
      661 ggccccattc cagagcgaat cctgggcaag atgactgtgg cgattgtgaa agcactgtac
      721 tatctgaagg agaagcatgg cgtcatccat cgcgatgtca aaccctccaa catcctgcta
      781 gatgagcggg gccagatcaa gctctgtgac tttggcatca gtggccgcct tgttgactcc
      841 aaagccaaaa cacggagtgc tggctgtgct gcctatatgg ctcccgagcg catcgaccct
      901 ccagatccca ccaagcctga ctatgacatc cgagctgatg tgtggagcct gggcatctca
      961 ctggtggagc tggcaacagg acagttcccc tataagaact gcaagacgga ctttgaggtc
     1021 ctcaccaaag tcctacagga agagccccca ctcctgcctg gtcacatggg cttctcaggg
     1081 gacttccagt catttgtcaa agactgcctt actaaagatc acaggaagag accaaagtat
     1141 aataagctac ttgaacacag cttcatcaag cactatgaga tactcgaggt ggatgtcgcg
     1201 tcctggttta aggatgtcat ggcgaagacc gagtccccaa ggactagtgg agtcctgagt
     1261 cagcaccatc tgcccttctt cagtgggagt ctggaggagt ctcccacttc cccaccttct
     1321 cccaagtcct tccctctgtc accagccatc cctcacgaaa ttctgagccg tcgtccgagt
     1381 taccgtaaag gatccatgag cgacagtaaa tgtgacagtc agttttatag tgtgcaagtg
     1441 gcagactcaa ccttcactgt cctaaaacgt taccagcagc tgaaaccaat tggctctggg
     1501 gcccaaggga ttgtttgtgc tgcatttgat acagttcttg ggataaatgt tgcagtcaag
     1561 aaactaagcc gtccttttca gaaccaaact catgcaaaga gagcttatcg tgaacttgtc
     1621 ctcttaaaat gtgtcaatca taaaaatata attagtttgt taaatgtgtt tacaccacaa
     1681 aaaactctag aagaatttca agatgtgtat ttggttatgg aattaatgga tgctaactta
     1741 tgtcaggtta ttcacatgga gctggatcat gaaagaatgt cctaccttct ttaccagatg
     1801 ctttgtggta ttaaacatct gcattcagct ggtataattc atagagattt gaagcctagc
     1861 aacattgttg tgaaatcaga ctgcaccctg aagatccttg actttggcct ggcccggaca
     1921 gcgtgcacta acttcatgat gaccccttac gtggtgacac ggtactaccg ggcgcccgaa
     1981 gtcatcctgg gtatgggcta caaagagaac gttgatatct ggtcagtggg ttgcatcatg
     2041 ggagagctgg tgaaaggttg tgtgatattc caaggcactg accatattga tcagtggaat
     2101 aaagttattg agcagctggg aacaccatca gcagagttca tgaagaaact tcagccaact
     2161 gtgaggaatt atgtcgaaaa cagaccaaag tatcctggaa tcaaatttga agaactcttt
     2221 ccagattgga tattcccatc agaatctgag cgagacaaaa taaaaacaag tcaagccaga
     2281 gatctgttat caaaaatgtt agtgattgat cctgacaagc ggatctctgt agacgaagct
     2341 ctgcgtcacc catacatcac tgtttggtat gaccccgccg aagcag
//
