LOCUS       sequence-108725-         987 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses drosophila IMD tagged with V5 and 6xHis epitopes, under 
            control of metallothionein promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 987)
  TITLE     pMT-IMD-V5-His
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 987)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMT-IMD-V5-His
FEATURES             Location/Qualifiers
     source          1..987
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    146..280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(183..202)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     237..256
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     282..299
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(322..339)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(349..390)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 accatgatta cgccaagctt gcatgcctgc aggtcgactg atcataatca gccataccac
       61 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
      121 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
      181 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
      241 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc ttagaaggca cagtcgaggc
      301 tgatcagcgg gtttaaactc aatggtgatg gtgatgatga ccggtacgcg tagaatcgag
      361 accgaggaga gggttaggga taggcttacc ttcgaagggc cctctagact cgaggctgtt
      421 tgtcttgcgc ttctccagtg ccttccaaac cagtttcatg cgctgcacgc agtcgcgatg
      481 ctgggactcc cacagcagtg tagtgagtcg tccaacggtg gccacaccgt ccgccgagct
      541 tcgaatccac tggagcaaca gctggtatat cacctctcta atattgccat gcatttggta
      601 atcgattatg gcctggtcga tctgtccttc cgacatgcca agatcccgca tcacctgctt
      661 ccagccctcg cccaagtgcg tggaaaccac atcgagcagg cgcacatccg gttcctcttg
      721 tgactgcatc atggccacaa tgctgaccgt tttgcgcgtc gcacttgccc gcggcttacc
      781 gtctggagag gcgaccgact cctctgcggt actggtgcta ccctttcgcg agctgcaggc
      841 gctcaagttt tggttgaagt tgtagacgga gccgaagtgc agattattgg cattcgagaa
      901 gttcatgacc acctgttgct gggcgctgtg tacgttcatg gtgttgtttg tctggacgtt
      961 actgagagtg cccggaatat tggcgga
//
