LOCUS       sequence-108755-         758 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV 
            promoter and iRFP module. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 758)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV iRFP L3-L2
FEATURES             Location/Qualifiers
     source          1..758
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..46
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        27..45
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      80..89
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttgg
       61 taccgagctc ggatctgccg ccaccatggc ggaaggatcc gtcgccaggc agcctgacct
      121 cttgacctgc gacgatgagc cgatccatat ccccggtgcc atccaaccgc atggactgct
      181 gctcgccctc gccgccgaca tgacgatcgt tgccggcagc gacaaccttc ccgaactcac
      241 cggactggcg atcggcgccc tgatcggccg ctctgcggcc gatgtcttcg actcggagac
      301 gcacaaccgt ctgacgatcg ccttggccga gcccggggcg gccgtcggag caccgatcac
      361 tgtcggcttc acgatgcgaa aggacgcagg cttcatcggc tcctggcatc gccatgatca
      421 gctcatcttc ctcgagctcg agcctcccca gcgggacgtc gccgagccgc aggcgttctt
      481 ccgccgcacc aacagcgcca tccgccgcct gcaggccgcc gaaaccttgg aaagcgcctg
      541 cgccgccgcg gcgcaagagg tgcggaagat taccggcttc gatcgggtga tgatctatcg
      601 cttcgcctcc gacttcagcg gcgaagtgat cgcagaggat cggtgcgccg aggtcgagtc
      661 aaaactaggc ctgcactatc ctgcctcaac cgtgccggcg caggcccgtc ggctctatac
      721 catcaacccg gtacggatca ttcccgatat caattatc
//
