LOCUS       sequence-108756-         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV 
            promoter and iRFP module. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV iRFP L5-L2
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actgcttact ggcttatcga aattaatacg actcactata gggagaccca agcttggtac
       61 cgagctcgga tctgccgcca ccatggcgga aggatccgtc gccaggcagc ctgacctctt
      121 gacctgcgac gatgagccga tccatatccc cggtgccatc caaccgcatg gactgctgct
      181 cgccctcgcc gccgacatga cgatcgttgc cggcagcgac aaccttcccg aactcaccgg
      241 actggcgatc ggcgccctga tcggccgctc tgcggccgat gtcttcgact cggagacgca
      301 caaccgtctg acgatcgcct tggccgagcc cggggcggcc gtcggagcac cgatcactgt
      361 cggcttcacg atgcgaaagg acgcaggctt catcggctcc tggcatcgcc atgatcagct
      421 catcttcctc gagctcgagc ctccccagcg ggacgtcgcc gagccgcagg cgttcttccg
      481 ccgcaccaac agcgccatcc gccgcctgca ggccgccgaa accttggaaa gcgcctgcgc
      541 cgccgcggcg caagaggtgc ggaagattac cggcttcgat cgggtgatga tctatcgctt
      601 cgcctccgac ttcagcggcg aagtgatcgc agaggatcgg tgcgccgagg tcgagtcaaa
      661 actaggcctg cactatcctg cctcaaccgt gccggcgcag gcccgtcggc tctataccat
      721 caacccggta cggatcattc ccgatatcaa ttatcggccg gtgccggtca
//
