LOCUS       sequence-108758-         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of Chronos-tdTomato under the Syn promoter. 
            Using bGHpA signal. tdTomato has codons varied between the first and
            second tandem repeats to reduce recombination..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Klapoetke NC, Murata Y, Kim SS, Pulver SR, Birdsey-Benson A, Cho YK,
            Morimoto TK, Chuong AS, Carpenter EJ, Tian Z, Wang J, Xie Y, Yan Z, 
            Zhang Y, Chow BY, Surek B, Melkonian M, Jayaraman V, 
            Constantine-Paton M, Wong GK, Boyden ES
  TITLE     Independent optical excitation of distinct neural populations.
  JOURNAL   Nat Methods. 2014 Mar;11(3):338-46. doi: 10.1038/nmeth.2836. Epub 
            2014 Feb 9.
  PUBMED    24509633
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2836"; journalName: "Nat Methods"; date: "2014-03"; 
            volume: "11"; issue: "3"; pages: "338-46"
COMMENT     Sequence Label: pAAV-Syn-Chronos-tdTomato
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgtcgtgcc tgagagcgca gtcgagagga tccgccacca tggaaacagc cgccacaatg
       61 acccacgcct ttatctcagc cgtgcctagc gccgaagcca caattagagg cctgctgagc
      121 gccgcagcag tggtgacacc agcagcagac gctcacggag aaacctctaa cgccacaaca
      181 gccggagccg atcacggttg cttcccccac atcaaccacg gaaccgagct gcagcacaag
      241 atcgcagtgg gactccagtg gttcaccgtg atcgtggcta tcgtgcagct catcttctac
      301 ggttggcaca gcttcaaggc cacaaccggc tgggaggagg tctacgtctg cgtgatcgag
      361 ctcgtcaagt gcttcatcga gctgttccac gaggtcgaca gcccagccac agtgtaccag
      421 accaacggag gagccgtgat ttggctgcgg tacagcatgt ggctcctgac ttgccccgtg
      481 atcctgatcc acctgagcaa cctgaccgga ctgcacgaag agtacagcaa gcggaccatg
      541 accatcctgg tgaccgacat cggcaacatc gtgtggggga tcacagccgc ctttacaaag
      601 ggccccctga agatcctgtt cttcatgatc ggcctgttct acggcgtgac ttgcttcttc
      661 cagatcgcca aggtgtatat cgagagctac cacaccctgc ccaaaggcgt ctgccggaag
      721 atttgcaaga tcatggccta cgtcttcttc tgctcttggc tgatgttccc cgtgatgttc
      781 atcgccggac acgagggact gggcctgatc acaccttaca ccagcggaat cggccacctg
      841 atcctggatc tgatcagcaa gaacacttgg ggc
//
