LOCUS       sequence-108767-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TVA66T-EGFP under the synapsin promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-synP-FLEX-TVA66T-EGFP
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(4..37)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(88..121)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     primer_bind     complement(173..194)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     522..541
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     781..802
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttcataactt cgtatagcat acattatacg aagttattct ttgcaccatt ctaaagaata
       61 acagtgataa tttctgggtt aaggcaaata acttcgtata ggatacttta tacgaagtta
      121 tgctagctca cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
      181 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
      241 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      301 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
      361 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
      421 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
      481 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
      541 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
      601 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
      661 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
      721 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
      781 cgtcgccgtc cagctcgacc aggatg
//
