LOCUS       sequence-108768-         722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TVA66T-EGFP-B19G under the synapsin promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 722)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-synP-FLEX-TVA66T-EGFP-B19G
FEATURES             Location/Qualifiers
     source          1..722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(14..47)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(98..131)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 cgcagtcgaa ttcataactt cgtatagcat acattatacg aagttattct ttgcaccatt
       61 ctaaagaata acagtgataa tttctgggtt aaggcaaata acttcgtata ggatacttta
      121 tacgaagtta tgctagctca cagtctggtc tcacccccac tcttgtgtga ttcccatgaa
      181 gatatgatct tcccgctttg gggagtgact gacacctccc tccctgtccc tctgagattg
      241 tgttgcgtag gttctgatcg attgactctt ctacaacatg tcatcaggaa aattatcaac
      301 atcaaggcag tcagggcccc tgcactcagt aatacatact tcccccagtt cgggagaccc
      361 aagtcaactc ctgagacctg attgtgcaca tcgggaaggt gaacttcaac aaaatcctca
      421 gcctcgtcac cgtccttgaa aacggtagac gggtctgcca gggggtgcac aagggggata
      481 accgaggatt ccaacaactc catatgttgc tggaggaggg atgattgcat ctctgggatt
      541 aagacattgc cgtcaggtcc taatattata ccattgaaaa acaccccgtt cacatgagga
      601 tgacacctcc ccccaactct taaacaccct tttgaaggga ggatctcatt ccaagttctg
      661 actgacttgt agtgagcatc ggcttccatc aaggtcttgt tgaatatggt atatgctttt
      721 cc
//
