LOCUS       sequence-108770-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) 
            preceded by a MYR signal and a restriction site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  TITLE     MYR-VfAU1-LOV_135
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MYR-VfAU1-LOV_135
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..147
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             622..648
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(725..742)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cacaagctgg
       61 ctagcgttta aacgggccct ctagactcga gcggccgcgg ccaccatggg gagtagcaag
      121 agcaagccta aggaccccag ccagcgcctc gacgtcaccg gtggacctga ctacagtctc
      181 gtgaaggctc tgcaaatggc acaacagaat tttgtcatta cagacgcctc cctcccagac
      241 aaccctatcg tctacgccag tagagggttt ctgacactga caggctattc tctcgaccag
      301 atcctgggca ggaactgcag gtttctgcaa gggccagaaa cagacccaag agctgtggat
      361 aagatcagga atgccatcac caaaggcgtt gataccagtg tctgtctgct gaattataga
      421 caggatggca caaccttctg gaatctcttc ttcgtggctg gactcagaga ttctaagggc
      481 aatattgtca actacgtcgg agtgcagtca aaggtgagcg aagattatgc caagctgctg
      541 gtcaacgagc agaacattga gtacaaaggt gtgcgcacca gtaacatgct gcgcagaaag
      601 cccggtggat ccggagtcga ctatccgtac gacgtaccag actacgcact cgactaagaa
      661 ttccaccaca ctggactagt ggatccgagc tcggtaccaa gcttaagttt aaaccgctga
      721 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
      781 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataa
//
