LOCUS       sequence-108772-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by a 
            restriction site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  TITLE     MYR-VfAU1-LOV_135
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: VfAU1-LOV_136
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..51
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        32..50
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             573..599
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(676..693)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    682..793
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg gagacacaag
       61 ctggctagcg tttaaacggg ccctctagac tcgagcggcc gcggcccacc ggtggacctg
      121 actacagtct cgtgaaggct ctgcaaatgg cacaacagaa ttttgtcatt acagacgcct
      181 ccctcccaga caaccctatc gtctacgcca gtagagggtt tctgacactg acaggctatt
      241 ctctcgacca gatcctgggc aggaactgca ggtttctgca agggccagaa acagacccaa
      301 gagctgtgga taagatcagg aatgccatca ccaaaggcgt tgataccagt gtctgtctgc
      361 tgaattatag acaggatggc acaaccttct ggaatctctt cttcgtggct ggactcagag
      421 attctaaggg caatattgtc aactacgtcg gagtgcagtc aaaggtgagc gaagattatg
      481 ccaagctgct ggtcaacgag cagaacattg agtacaaagg tgtgcgcacc agtaacatgc
      541 tgcgcagaaa gcccggtgga tccggagtcg actatccgta cgacgtacca gactacgcac
      601 tcgactaaga attccaccac actggactag tggatccgag ctcggtacca agcttaagtt
      661 taaaccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
      721 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
      781 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggg
//
