LOCUS       sequence-108773-         833 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  yeast Cox15 heme A synthase with C-terminal 13xMyc tag in low-copy 
            yeast expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Swenson S, Cannon A, Harris NJ, Taylor NG, Fox JL, Khalimonchuk O
  TITLE     Analysis of Oligomerization Properties of Heme a Synthase Provides 
            Insights into its Function in Eukaryotes.
  JOURNAL   J Biol Chem. 2016 Mar 3. pii: jbc.M115.707539.
  PUBMED    26940873
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Mar 3. pii: jbc.M115.707539."
COMMENT     Sequence Label: pRS415-Cox15-13xMyc
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(81..268)
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     189..213
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             complement(323..820)
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
ORIGIN
        1 gggacaaaag ctggagctcc accgcggtgg cggccgctct agaactagtg tatattaccc
       61 tgttatccct agcggatctg ccggtagagg tgtggtcaat aagagcgacc tcatactata
      121 cctgagaaag caacctgacc tacaggaaag agttactcaa gaataagaat tttcgtttta
      181 aaacctaaga gtcactttaa aatttgtata cacttatttt ttttataact tatttaataa
      241 taaaaatcat aaatcataag aaattcgctt atttagaagt ggcgcgaatt cactagtgat
      301 tgattaattt ttgttcaccg ttcaagtctt cctcggagat tagcttttgt tcaccgttca
      361 aatcttcttc agaaatcaac ttttgttcac cgtcgagtcc gttcaagtct tcttctgaga
      421 ttaatttttg ttcaccgttc aagtcttcct cggagattag cttttgttca ccgttcaaat
      481 cttcttcaga aatcaacttt tgttcaccgt cgagtccgtt caagtcttct tctgagatta
      541 atttttgttc accgttcaag tcttcctcgg agattagctt ttgttcaccg ttcaaatctt
      601 cttcagaaat caacttttgt tcaccgtcga gtccgttcaa gtcttcttct gagattaatt
      661 tttgttcacc gttcaagtct tcctcggaga ttagcttttg ttcaccgttc aaatcttctt
      721 cagaaatcaa cttttgttca ccgtcgagtc cgttcaagtc ttcttctgag attaattttt
      781 gttcaccgtt caagtcttcc tcggagatta gcttttgttc accgttaatt aac
//
