LOCUS       sequence-108775-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) 
            preceded by the extracellular and transmembrane domain of p75NTR and
            a restriction site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  TITLE     MYR-VfAU1-LOV_135
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: P75ECD-VfAU1-LOV_448
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      108..117
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcgttta aacgggccct ctagactcga gcggccgcgg ccggcgcgcc accatgggag
      121 caggtgcaac tggtagagca atggacggac ctagactcct gctgctcctc ctgctcggag
      181 tgtccctggg tggagcaaaa gaagcatgcc caactggact ctacacccat tctggagagt
      241 gctgcaaggc ctgcaatctg ggtgaaggag tcgctcagcc atgtggagca aaccagaccg
      301 tctgtgagcc ttgcctggat tctgtgacat tctccgacgt ggtgtctgcc accgagcctt
      361 gcaagccatg caccgagtgc gttggcctgc agtcaatgtc tgctccctgc gtggaggctg
      421 acgacgcagt gtgtagatgc gcatatggct actaccagga tgaaactact gggaggtgcg
      481 aggcatgtag ggtgtgtgag gcaggcagtg gtctggtttt cagttgccag gacaagcaga
      541 acaccgtctg cgaggagtgt ccagacggga catacagcga cgaagccaac catgtcgatc
      601 cctgcctgcc atgtaccgtt tgcgaagata ccgaacgcca gctgagagaa tgcactagat
      661 gggctgacgc cgaatgtgag gagatcccag gacggtggat tactcgctct actcctccag
      721 aagggtcaga tagcaccgct cccagcaccc aagaacccga agctcctccc gagcaggatc
      781 tgattgcctc cacagttgct ggagtcgtga ctaccgtgat gg
//
