LOCUS       sequence-108779-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TVA66T-EGFP under the CAG promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-CAG-FLEX-TVA66T-EGFP
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(21..54)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(105..138)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      147..156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(633..654)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 atccagaggt tgattccgga ataacttcgt ataggatact ttatacgaag ttatgcagaa
       61 tggtagctgg attgtagctg ctattagcaa tatgaaacct cttaataact tcgtatagca
      121 tacattatac gaagttatgg cgcgccgcca ccatggcgcg gctgctgccc gcgctgctgc
      181 tgctgctgct gcccggtaac gtgaccggta acgggtccgg taacggttct ttgtcccgtt
      241 gcccccccgg tcagttccgc tgctcggagc cgcccggtgc ccacggggag tgttacccgc
      301 aggactggct gtgcgacgga caccccgact gcgacgacgg gcgggacacc tggggctgcg
      361 ggaccagcgc gatccccgcg gtgcccacgg acaacggcac agaggctccc actgcccctg
      421 ctcctggacg tgctctgcca gccaggaatc acggccgcat gtggatgctg atcactgcag
      481 tgctcctgtg ctgcctggta gctgtgggtg gtatcgctgc atgggggaag tccaaagcaa
      541 aaagcaggtc tgacatcttc agtcttgaaa gcgcatccaa ggggggtggg gtgagcaagg
      601 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      661 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      721 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      781 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      841 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacg
//
