LOCUS       sequence-108787-         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses TVA66T-EGFP-B19G under the synapsin promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV-synP-FLEX-TVA66T-EGFP-B19G
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(29..62)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(113..146)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      155..164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(641..662)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 attttgtaat ccagaggttg attccggaat aacttcgtat aggatacttt atacgaagtt
       61 atgcagaatg gtagctggat tgtagctgct attagcaata tgaaacctct taataacttc
      121 gtatagcata cattatacga agttatggcg cgccgccacc atggcgcggc tgctgcccgc
      181 gctgctgctg ctgctgctgc ccggtaacgt gaccggtaac gggtccggta acggttcttt
      241 gtcccgttgc ccccccggtc agttccgctg ctcggagccg cccggtgccc acggggagtg
      301 ttacccgcag gactggctgt gcgacggaca ccccgactgc gacgacgggc gggacacctg
      361 gggctgcggg accagcgcga tccccgcggt gcccacggac aacggcacag aggctcccac
      421 tgcccctgct cctggacgtg ctctgccagc caggaatcac ggccgcatgt ggatgctgat
      481 cactgcagtg ctcctgtgct gcctggtagc tgtgggtggt atcgctgcat gggggaagtc
      541 caaagcaaaa agcaggtctg acatcttcag tcttgaaagc gcatccaagg ggggtggggt
      601 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
      661 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
      721 gctgaccctg aagttcatct gcacca
//
