LOCUS       sequence-108789-         812 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) 
            preceded by a MYR signal and a restriction site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 812)
  TITLE     MYR-VfAU1-LOV_135
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 812)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: MYR-VfAU1-LOV_135
FEATURES             Location/Qualifiers
     source          1..812
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..68)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(543..584)
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     regulatory      581..590
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(643..662)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(644..662)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(707..806)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(736..756)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctcggatcca ctagtccagt gtggtggaat tcttagtcga gtgcgtagtc tggtacgtcg
       61 tacggatagt cgactccgga tccaccgggc tttctgcgca gcatgttact ggtgcgcaca
      121 cctttgtact caatgttctg ctcgttgacc agcagcttgg cataatcttc gctcaccttt
      181 gactgcactc cgacgtagtt gacaatattg cccttagaat ctctgagtcc agccacgaag
      241 aagagattcc agaaggttgt gccatcctgt ctataattca gcagacagac actggtatca
      301 acgcctttgg tgatggcatt cctgatctta tccacagctc ttgggtctgt ttctggccct
      361 tgcagaaacc tgcagttcct gcccaggatc tggtcgagag aatagcctgt cagtgtcaga
      421 aaccctctac tggcgtagac gatagggttg tctgggaggg aggcgtctgt aatgacaaaa
      481 ttctgttgtg ccatttgcag agccttcacg agactgtagt caggtccacc ggtgacgtcg
      541 aggcgctggc tggggtcctt aggcttgctc ttgctactcc ccatggtggc cgcggccgct
      601 cgagtctaga gggcccgttt aaacgctagc cagcttgtgt ctccctatag tgagtcgtat
      661 taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat
      721 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
      781 attttggaaa gtcccgttga ttttggtgcc aa
//
