LOCUS       sequence-108792-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  VfAU1-LOV domain (Vaucheria frigida Aureochrome 1) preceded by a 
            restriction site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  TITLE     MYR-VfAU1-LOV_135
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: VfAU1-LOV_136
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(32..58)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(580..599)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(581..599)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(644..743)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(673..693)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctagtccagt gtggtggaat tcttagtcga gtgcgtagtc tggtacgtcg tacggatagt
       61 cgactccgga tccaccgggc tttctgcgca gcatgttact ggtgcgcaca cctttgtact
      121 caatgttctg ctcgttgacc agcagcttgg cataatcttc gctcaccttt gactgcactc
      181 cgacgtagtt gacaatattg cccttagaat ctctgagtcc agccacgaag aagagattcc
      241 agaaggttgt gccatcctgt ctataattca gcagacagac actggtatca acgcctttgg
      301 tgatggcatt cctgatctta tccacagctc ttgggtctgt ttctggccct tgcagaaacc
      361 tgcagttcct gcccaggatc tggtcgagag aatagcctgt cagtgtcaga aaccctctac
      421 tggcgtagac gatagggttg tctgggaggg aggcgtctgt aatgacaaaa ttctgttgtg
      481 ccatttgcag agccttcacg agactgtagt caggtccacc ggtgggccgc ggccgctcga
      541 gtctagaggg cccgtttaaa cgctagccag cttgtgtctc cctatagtga gtcgtattaa
      601 tttcgataag ccagtaagca gtgggttctc tagttagcca gagagctctg cttatataga
      661 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
      721 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
      781 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaa
//
