LOCUS       sequence-108811-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK 
            RNA polymerase III promoter and miR30-based hairpin module for shRNA
            expression. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR 7SK-miR-30 L5-L2
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..79
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     ncRNA           394..487
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           501..613
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(625..724)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gactgatagt gacctgtttc gttgcaacaa attgatgagc aatgcttttt tataatgcca
       61 actttgtata caaaagttgc tatcgatcta gtgctgcagt atttagcatg ccccacccat
      121 ctgcaaggca ttctggatag tgtcaaaaca gccggaaatc aagtccgttt atctcaaact
      181 ttagcatttt gggaataaat gatatttgct atgctggtta aattagattt tagttaaatt
      241 tcctgctgaa gctctagtac gataagtaac ttgacctaag tgtaaagttg agatttcctt
      301 caggtttata tagcttgtgc gccgcctggg tacctcggtc ttcacagatc tgcagcacat
      361 atactagtcg actagggata acagggtaat tgtttgaatg aggcttcagt actttacaga
      421 atcgttgcct gcacatcttg gaaacacttg ctgggattac ttcttcaggt taacccaaca
      481 gaaggctcga gcaaccagaa ttcaaggggc tactttagga gcaattatct tgtttactaa
      541 aactgaatac cttgctatct ctttgataca tttttacaaa gctgaattaa aatggtataa
      601 attaaatcac ttttttcaat tcgtacccag ctttcttgta caaagttggc attataagaa
      661 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta
      721 tttgccatcc a
//
