LOCUS       sequence-108816-         669 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK 
            RNA polymerase III promoter and miR30-based hairpin module for shRNA
            expression. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 669)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 669)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR 7SK-miR-30 L5-L4
FEATURES             Location/Qualifiers
     source          1..669
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    73..93
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     ncRNA           complement(108..220)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(234..327)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(642..662)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat aagcaatgct
       61 ttcttataat gccaactttg tatagaaaag ttgggtgcga attgaaaaaa gtgatttaat
      121 ttataccatt ttaattcagc tttgtaaaaa tgtatcaaag agatagcaag gtattcagtt
      181 ttagtaaaca agataattgc tcctaaagta gccccttgaa ttctggttgc tcgagccttc
      241 tgttgggtta acctgaagaa gtaatcccag caagtgtttc caagatgtgc aggcaacgat
      301 tctgtaaagt actgaagcct cattcaaaca attaccctgt tatccctagt cgactagtat
      361 atgtgctgca gatctgtgaa gaccgaggta cccaggcggc gcacaagcta tataaacctg
      421 aaggaaatct caactttaca cttaggtcaa gttacttatc gtactagagc ttcagcagga
      481 aatttaacta aaatctaatt taaccagcat agcaaatatc atttattccc aaaatgctaa
      541 agtttgagat aaacggactt gatttccggc tgttttgaca ctatccagaa tgccttgcag
      601 atgggtgggg catgctaaat actgcagcac tagatcgata gcaacttttg tatacaaagt
      661 tggcattat
//
