LOCUS       sequence-108817-        5128 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pKT vector encoding yeast-codon optimized infrared fluorescent 
            protein iRFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Hansen AS, O'Shea EK
  TITLE     Promoter decoding of transcription factor dynamics involves a 
            trade-off between noise and control of gene expression.
  JOURNAL   Mol Syst Biol. 2013 Nov 5;9:704. doi: 10.1038/msb.2013.56.
  PUBMED    24189399
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2013-11-5"; volume: "9:704. doi"; pages: " 
            10.1038/msb.2013.56"
COMMENT     Sequence Label: pKT-iRFP-KAN
FEATURES             Location/Qualifiers
     source          1..5128
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      976..1163
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1031..1055)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     gene            1238..2594
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        1238..1581
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             1582..2391
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1649..1668)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     terminator      2397..2594
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(2698..2717)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2699..2717)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2804..2821)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2975..3563)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3055..3074)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3734..4525,4526..4594))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4357..4376
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4595..4699)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4767..4785
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4823..4845)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4945..4964
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        5045..5063
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5045..5062
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 taacatggct gaaggttctg ttgctagaca accagacttg ttgacttgtg acgacgaacc
       61 aatccacatc ccaggtgcta tccaaccaca cggtttgttg ttggctttgg ctgctgacat
      121 gactatcgtt gctggttctg acaacttgcc agaattgact ggtttggcta tcggtgcttt
      181 gatcggtaga tctgctgctg acgttttcga ctctgaaact cacaacagat tgactatcgc
      241 tttggctgaa ccaggtgctg ctgttggtgc tccaatcact gttggtttca ctatgagaaa
      301 ggacgctggt ttcatcggtt cttggcacag acacgaccaa ttgatcttct tggaattgga
      361 accaccacaa agagacgttg ctgaaccaca agctttcttc agaagaacta actctgctat
      421 cagaagattg caagctgctg aaactttgga atctgcttgt gctgctgctg ctcaagaagt
      481 tagaaagatc actggtttcg acagagttat gatctacaga ttcgcttctg acttctctgg
      541 tgaagttatc gctgaagaca gatgtgctga agttgaatct aagttgggtt tgcactaccc
      601 agcttctact gttccagctc aagctagaag attgtacact atcaacccag ttagaatcat
      661 cccagacatc aactacagac cagttccagt tactccagac ttgaacccag ttactggtag
      721 accaatcgac ttgtctttcg ctatcttgag atctgtttct ccagttcact tggaattcat
      781 gagaaacatc ggtatgcacg gtactatgtc tatctctatc ttgagaggtg aaagattgtg
      841 gggtttgatc gtttgtcacc acagaactcc atactacgtt gacttggacg gtagacaagc
      901 ttgtgaattg gttgctcaag ttttggcttg gcaaatcggt gttatggaag aataaggcgc
      961 gccacttcta aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata
     1021 aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta
     1081 ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagta tgaggtcgct
     1141 cttattgacc acacctctac cggcagatcc gctagggata acagggtaat atagatctgt
     1201 ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct
     1261 ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta
     1321 gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg
     1381 aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga
     1441 cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc
     1501 actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat
     1561 cacatccgaa cataaacaac catgggtaag gaaaagactc acgtttcgag gccgcgatta
     1621 aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa
     1681 tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt gtttctgaaa
     1741 catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg
     1801 acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg
     1861 ttactcacca ctgcgatccc cggcaaaaca gcattccagg tattagaaga atatcctgat
     1921 tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct
     1981 gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga
     2041 atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt
     2101 gaacaagtct ggaaagaaat gcataagctt ttgccattct caccggattc agtcgtcact
     2161 catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt
     2221 gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc
     2281 ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat
     2341 cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta atcagtactg
     2401 acaataaaaa gattcttgtt ttcaagaact tgtcatttgt atagtttttt tatattgtag
     2461 ttgttctatt ttaatcaaat gttagcgtga tttatatttt ttttcgcctc gacatcatct
     2521 gcccagatgc gaagttaagt gcgcagaaag taatatcatg cgtcaatcgt atgtgaatgc
     2581 tggtcgctat actgctgtcg attcgatact aacgccgcca tccagtttaa acgagctcga
     2641 attcatcgat gatatcagat ccactagtgg cctatgcggc cgcggatctg ccggtctccc
     2701 tatagtgagt cgtattaatt tcgataagcc aggttaacct gcattaatga atcggccaac
     2761 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     2821 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     2881 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     2941 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     3001 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     3061 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     3121 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct
     3181 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     3241 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     3301 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     3361 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     3421 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     3481 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     3541 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     3601 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     3661 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     3721 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     3781 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     3841 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     3901 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     3961 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     4021 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     4081 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     4141 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     4201 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     4261 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     4321 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     4381 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     4441 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     4501 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     4561 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     4621 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     4681 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     4741 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     4801 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     4861 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     4921 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     4981 tggacatatt gtcgttagaa cgcggctaca attaatacat aaccttatgt atcatacaca
     5041 tacgatttag gtgacactat agaacgcggc cgccagctga agcttcgtac gctgcaggtc
     5101 gacggatcgg tgacggtgct ggtttaat
//
