LOCUS       sequence-108821-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK 
            RNA polymerase III promoter and miR30-based hairpin module for shRNA
            expression. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR 7SK-miR-30 L1-L4
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     ncRNA           396..489
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           503..615
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(630..725)
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(756..775)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(757..775)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtacaa aaaagcaggc tctatcgatc tagtgctgca gtatttagca tgccccaccc
      121 atctgcaagg cattctggat agtgtcaaaa cagccggaaa tcaagtccgt ttatctcaaa
      181 ctttagcatt ttgggaataa atgatatttg ctatgctggt taaattagat tttagttaaa
      241 tttcctgctg aagctctagt acgataagta acttgaccta agtgtaaagt tgagatttcc
      301 ttcaggttta tatagcttgt gcgccgcctg ggtacctcgg tcttcacaga tctgcagcac
      361 atatactagt cgactaggga taacagggta attgtttgaa tgaggcttca gtactttaca
      421 gaatcgttgc ctgcacatct tggaaacact tgctgggatt acttcttcag gttaacccaa
      481 cagaaggctc gagcaaccag aattcaaggg gctactttag gagcaattat cttgtttact
      541 aaaactgaat accttgctat ctctttgata catttttaca aagctgaatt aaaatggtat
      601 aaattaaatc acttttttca attcgcaccc aacttttcta tacaaagttg gcattataag
      661 aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa ataaaatcat
      721 tatttgccat ccagctgcag ggcggccgcg atatccccta tagtgagtcg tattacatgg
      781 tcatagctg
//
