LOCUS       sequence-108822-         739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a 7SK 
            RNA polymerase III promoter and miR30-based hairpin module for shRNA
            expression. Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR 7SK-miR-30 L1-L4
FEATURES             Location/Qualifiers
     source          1..739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    15..110
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           complement(125..237)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(251..344)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    complement(659..683)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tgcagctgga tggcaaataa tgattttatt ttgactgata gtgacctgtt cgttgcaaca
       61 aattgataag caatgctttc ttataatgcc aactttgtat agaaaagttg ggtgcgaatt
      121 gaaaaaagtg atttaattta taccatttta attcagcttt gtaaaaatgt atcaaagaga
      181 tagcaaggta ttcagtttta gtaaacaaga taattgctcc taaagtagcc ccttgaattc
      241 tggttgctcg agccttctgt tgggttaacc tgaagaagta atcccagcaa gtgtttccaa
      301 gatgtgcagg caacgattct gtaaagtact gaagcctcat tcaaacaatt accctgttat
      361 ccctagtcga ctagtatatg tgctgcagat ctgtgaagac cgaggtaccc aggcggcgca
      421 caagctatat aaacctgaag gaaatctcaa ctttacactt aggtcaagtt acttatcgta
      481 ctagagcttc agcaggaaat ttaactaaaa tctaatttaa ccagcatagc aaatatcatt
      541 tattcccaaa atgctaaagt ttgagataaa cggacttgat ttccggctgt tttgacacta
      601 tccagaatgc cttgcagatg ggtggggcat gctaaatact gcagcactag atcgatagag
      661 cctgcttttt tgtacaaagt tggcattata aaaaagcatt gctcatcnat ttgttgcaac
      721 gaacaggtca ctatcagtc
//
