LOCUS       sequence-108824-         632 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 
            promoter and miR30-based hairpin module for shRNA expression. 
            Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 632)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR U6-miR-30 L5-L4
FEATURES             Location/Qualifiers
     source          1..632
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    28..48
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        113..361
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     113..133
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     284..303
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     ncRNA           416..509
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 ttgatgagca atgctttttt ataatgccaa ctttgtatac aaaagttggg caaaacgcac
       61 cacgtgacgg agcgtgaccg cgcgccgagc gcgcgccagg gtcgggcagg aagagggcct
      121 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattag
      181 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata
      241 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
      301 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
      361 cgtgctcgct tcggcagcac atatactagt cgactaggga taacagggta attgtttgaa
      421 tgaggcttca gtactttaca gaatcgttgc ctgcacatct tggaaacact tgctgggatt
      481 acttcttcag gttaacccaa cagaaggctc gagcaaccag aattcaaggg gctactttag
      541 gagcaattat cttgtttact aaaactgaat accttgctat ctctttgata catttttaca
      601 aagctgaatt aaaatggtat aaattaaatc ac
//
