LOCUS       sequence-108826-         439 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a U6 
            promoter and miR30-based hairpin module for shRNA expression. 
            Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR U6-miR-30 R4-R3
FEATURES             Location/Qualifiers
     source          1..439
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(11..135)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        199..439
                     /label=U6 promoter
                     /note="Truncated RNA polymerase III promoter for human U6 
                     snRNA used in pSIREN vectors such as 
                     pSIREN-RetroQ-ZsGreen1. Expected transcription start site 
                     is 8bp downstream of this feature."
     primer_bind     199..219
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     370..389
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 gtagttgatt catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
       61 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt
      121 tctatacaaa gttgggcaaa acgcaccacg tgacggagcg tgaccgcgcg ccgagcgcgc
      181 gccagggtcg ggcaggaaga gggcctattt cccatgattc cttcatattt gcatatacga
      241 tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa gatattagta
      301 caaaatacgt gacgtagaaa gtaataattt cttgggtaga ttgcagtttt aaaattatgt
      361 tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt cttggcttta
      421 tatatcttgt ggaaaggac
//
