LOCUS       sequence-108827-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 
            promoter and miR30-based hairpin module for shRNA expression. 
            Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR H1-miR-30 L1-R5
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        94..308
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     229..248
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     ncRNA           357..450
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           464..576
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     protein_bind    618..741
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(796..815)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(797..815)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(820..836)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(820..836)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
       61 ctttgtacaa aaaagcaggc tctatcgata ttcgaacgct gacgtcatca acccgctcca
      121 aggaatcgcg ggcccagtgt cactaggcgg gaacacccag cgcgcgtgcg ccctggcagg
      181 aagatggctg tgagggacag gggagtggcg ccctgcaata tttgcatgtc gctatgtgtt
      241 ctgggaaatc accataaacg tgaaatgtct ttggatttgg gaatcttata agttctgtat
      301 gagaccacag atctcggcgt tgtgtactag tcgactaggg ataacagggt aattgtttga
      361 atgaggcttc agtactttac agaatcgttg cctgcacatc ttggaaacac ttgctgggat
      421 tacttcttca ggttaaccca acagaaggct cgagcaacca gaattcaagg ggctacttta
      481 ggagcaatta tcttgtttac taaaactgaa taccttgcta tctctttgat acatttttac
      541 aaagctgaat taaaatggta taaattaaat cacttttttc aattggaaga ctaatgcgtt
      601 tatctagacc aattcgacaa ctttgtatac aaaagttgaa cgagaaacgt aaaatgatat
      661 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
      721 acaacatatc cagtcactat gaatcaacta cttagatggt attagtgacc tgtactgcag
      781 ggcggccgcg atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag
      841 ctct
//
