LOCUS       sequence-108829-         726 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a H1 
            promoter and miR30-based hairpin module for shRNA expression. 
            Compatible with MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR H1-miR-30 L1-R5
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(50..173)
                     /gene="mutant version of attR"
                     /label=attR5
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     ncRNA           complement(215..327)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(341..434)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        complement(483..697)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(543..562)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
ORIGIN
        1 cgcggccgcc ctgcagtaca ggtcactaat accatctaag tagttgattc atagtgactg
       61 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
      121 attgatattt atatcatttt acgtttctcg ttcaactttt gtatacaaag ttgtcgaatt
      181 ggtctagata aacgcattag tcttccaatt gaaaaaagtg atttaattta taccatttta
      241 attcagcttt gtaaaaatgt atcaaagaga tagcaaggta ttcagtttta gtaaacaaga
      301 taattgctcc taaagtagcc ccttgaattc tggttgctcg agccttctgt tgggttaacc
      361 tgaagaagta atcccagcaa gtgtttccaa gatgtgcagg caacgattct gtaaagtact
      421 gaagcctcat tcaaacaatt accctgttat ccctagtcga ctagtacaca acgccgagat
      481 ctgtggtctc atacagaact tataagattc ccaaatccaa agacatttca cgtttatggt
      541 gatttcccag aacacatagc gacatgcaaa tattgcaggg cgccactccc ctgtccctca
      601 cagccatctt cctgccaggg cgcacgcgcg ctgggtgttc ccgcctagtg acactgggcc
      661 cgcgattcct tggagcgggt tgatgacgtc agcgttcgaa tatcgataga gcctgctttt
      721 ttgtac
//
