LOCUS       sequence-10890-e        1707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-RIOK1
FEATURES             Location/Qualifiers
     source          1..1707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(188..205)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1347..1358
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atggactacc ggcggcttct catgagccgg gtggtccccg ggcaattcga cgacgcggac
       61 tcctctgaca gtgaaaacag agacttgaag acagtcaaag agaaggatga cattctgttt
      121 gaagaccttc aagacaatgt gaatgagaat ggtgaaggtg aaatagaaga tgaggaggag
      181 gagggttatg atgatgatga tgatgactgg gactgggatg aaggagttgg aaaactcgcc
      241 aagggttatg tctggaatgg aggaagcaac ccacaggcaa atcgacagac ctccgacagc
      301 agttcagcca aaatgtctac tccagcagac aaggtcttac ggaaatttga gaataaaatt
      361 aatttagata agctaaatgt tactgattcc gtcataaata aagtcaccga aaagtctaga
      421 caaaaggaag cagatatgta tcgcatcaaa gataaggcag acagagcaac tgtagaacag
      481 gtgttggatc ccagaacaag aatgatttta ttcaagatgt tgactagagg aatcataaca
      541 gagataaatg gctgcattag cacaggaaaa gaagctaatg tataccatgc tagcacagca
      601 aatggagaga gcagagcaat caaaatttat aaaacttcta ttttggtgtt caaagatcgg
      661 gataaatatg taagtggaga attcagattt cgtcatggct attgtaaagg aaaccctagg
      721 aaaatggtga aaacttgggc agaaaaagaa atgaggaact taatcaggct aaacacagca
      781 gagataccat gtccagaacc aataatgcta agaagtcatg ttcttgtcat gagtttcatc
      841 ggtaaagatg acatgcctgc accactcttg aaaaatgtcc agttatcaga atccaaggct
      901 cgggagttgt acctgcaggt cattcagtac atgagaagaa tgtatcagga tgccagactt
      961 gtccatgcag atctcagtga atttaacatg ctgtaccacg gtggaggcgt gtatatcatt
     1021 gacgtgtctc agtccgtgga gcacgaccac ccacatgcct tggagttctt gagaaaggat
     1081 tgcgccaacg tcaatgattt ctttatgagg cacagtgttg ctgtcatgac tgtgcgggag
     1141 ctctttgaat ttgtcacaga tccatccatt acacatgaga acatggatgc ttatctctca
     1201 aaggccatgg aaatagcatc tcaaaggacc aaggaagaac ggtctagcca agatcatgtg
     1261 gatgaagagg tgtttaagcg agcatatatt cctagaacct tgaatgaagt gaaaaattat
     1321 gagagggata tggacataat tatgaaattg aaggaagagg acatggccat gaatgcccaa
     1381 caagataata ttctatacca gactgttaca ggattgaaga aagatttgtc aggagttcag
     1441 aaggtccctg cactcctaga aaatcaagtg gaggaaagga cttgttctga ttcagaagat
     1501 attggaagct ctgagtgctc tgacacagac tctgaagagc agggagacca tgcccgcccc
     1561 aagaaacaca ccacggaccc tgacattgat aaaaaagaaa gaaaaaagat ggtcaaggaa
     1621 gcccagagag agaaaagaaa aaacaaaatt cctaaacatg tgaaaaaaag aaaggagaag
     1681 acagccaaga cgaaaaaagg caaatag
//
