LOCUS       sequence-108950-         721 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Lamp1 fused to YFP. Used for localization to
            the lysosome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Sherer NM, Lehmann MJ, Jimenez-Soto LF, Ingmundson A, Horner SM, 
            Cicchetti G, Allen PG, Pypaert M, Cunningham JM, Mothes W
  TITLE     Visualization of retroviral replication in living cells reveals 
            budding into multivesicular bodies.
  JOURNAL   Traffic 2003 Nov;4(11):785-801.
  PUBMED    14617360
REFERENCE   2  (bases 1 to 721)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic"; 
            date: "2003-11"; volume: "4(11)"; pages: "785-801"
COMMENT     Sequence Label: Lamp1-YFP
FEATURES             Location/Qualifiers
     source          1..721
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..231
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      273..282
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg ggtagcgggc
       61 gaagcactgc aggccgtagc cgaaggtggt cacgagggtg ggccagggca cgggcagctt
      121 gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc cctcgccctc
      181 gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca ggatgggcac
      241 caccccggtg aacagctcct cgcccttgct caccatggtg gcgaccggtg gatcccggat
      301 ggtctgatag cccgcgtgac tcctcttcct gccgatgagg taggcgatga ggacgatgag
      361 gaccagccct gccagggccc cgcccacagc aatggggatc agcatgttgt taccgtcctg
      421 tacacactct tccacagacc caaacctgtc actttctacc ctgaaagcct ggacttgcac
      481 gctgaagaca ttgagggcga gcgccttgct gacaaagatg tgctcctcac tgttgcactt
      541 gtatgagttg ccgacactgg cctgaagagc tttcagggaa tagttggagg tgctgaacgt
      601 gggctctatg gcatcaggaa gagtcatgtt caactgaact ccttgcagga aaaacaggct
      661 agaagtggca ttcatcccaa actgcagctc caggactctg ctcttgttcc ccaccttcag
      721 g
//
