LOCUS       sequence-109-exp        4463 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4463)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 4463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_85
FEATURES             Location/Qualifiers
     source          1..4463
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(274..302)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1050..1070
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(2037..2059)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2159..2178
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2256..2274)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2342..2446
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2447..2515,2516..3307)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2665..2684)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3478..4066
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3967..3986
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4220..4237
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4354..4375
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4390..4395,4396..4413,4414..4420)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4428..4444
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4433..4455
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(4452..4463,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(4452..4463,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggat
       61 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
      121 gaggaccctt ggctagcaaa aatgcataag gttttgctgg cactgttctt tatctttctg
      181 gcaccagcat ccgcactggc agtctccgaa ccggtcctag cgtcgacggt accggtagaa
      241 aaaatgagta aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
      301 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac
      361 ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc atgggtaagt
      421 ttaaacatat atatactaac taaccctgat tatttaaatt ttcagccaac acttgtcact
      481 actttcactt atggtgttca atgcttctcg agatacccag atcatatgaa acggcatgac
      541 tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat
      601 gacgggaact acaagacacg taagtttaaa cagttcggta ctaactaacc atacatattt
      661 aaattttcag gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
      721 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
      781 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttgtaagt
      841 ttaaacatga ttttactaac taactaatct gatttaaatt ttcagaactt caaaattaga
      901 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
      961 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     1021 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     1081 attacacatg gcatggatga actatacaaa tagcattcgt agaattccaa ctgagcgccg
     1141 gtcgctacca ttaccaactt gtctggtgtc aaaaataata ggggccgctg tcatcagagt
     1201 aagtttaaac tgagttctac taactaacga gtaatattta aattttcagc atctcgcgcc
     1261 cgtgcctctg acttctaagt ccaattactc ttcaacatcc ctacatgctc tttctccctg
     1321 tgctcccacc ccctattttt gttattatca aaaaacttct cttaatttct ttgtttttta
     1381 gcttctttta agtcacctct aacaatgaaa ttgtgtagat tcaaaaatag aattaattcg
     1441 taataaaaag tcgaaaaaaa ttgtgctccc tccccccatt aataataatt ctatcccaaa
     1501 atctacacaa tgttctgtgt acacttctta tgttttttac ttctgataaa tttttttgaa
     1561 acatcataga aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac
     1621 cgcaattttt atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg
     1681 aaaaagtttt ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt
     1741 tcctgctttt gctttttggg gtttccccta ttgtttgtca agatttcgag gacggcgttt
     1801 ttcttgctaa aatcacaagt attgatgagc acgatgcaag aaagatcgga agaaggtttg
     1861 ggtttgaggc tcagtggaag gtgagtagaa gttgataatt tgaaagtgga gtagtgtcta
     1921 tggggttttt gccttaaatg acagaataca ttcccaatat accaaacata actgtttcct
     1981 actagtcggc cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     2041 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     2101 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     2161 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc
     2221 gtaaggagaa aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata
     2281 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     2341 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     2401 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     2461 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     2521 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     2581 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     2641 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     2701 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     2761 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     2821 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     2881 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     2941 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     3001 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     3061 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     3121 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     3181 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     3241 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     3301 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     3361 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     3421 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     3481 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     3541 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     3601 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa
     3661 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     3721 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     3781 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     3841 caccgaactg agatacctac agcgtgagca ttgagaaagc gccacgcttc ccgaagggag
     3901 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     3961 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     4021 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     4081 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     4141 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     4201 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     4261 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     4321 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     4381 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     4441 acaatttcac acaggaaaca gct
//
