LOCUS       sequence-109041-       10312 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  generating transgenic TRIC flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10312)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 10312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pQUAST-p65AD::CaM
FEATURES             Location/Qualifiers
     source          1..10312
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3189..3427
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3374..3395
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             3517..3537
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3988..4359
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             4402..4845
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     intron          4941..5006
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             5136..5156
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    5428..5562
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5465..5484)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5519..5538
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5589..5607)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6067..6138,6533..6806,6881..7535,7597..7912,8133..8264,
                     8335..8949)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(9727..10281,10282..10312)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt ggatccgggt aatcgcttat
     3061 cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc
     3121 tcgggtaatc gcttatcctt aagcttgata tcgaattcct gcagcccgag cggagactct
     3181 agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     3241 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     3301 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
     3361 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag
     3421 aactctgaat agggaattgg gaattcgtta acagatcggg ggatcaattc gttaacagat
     3481 ctcaacatgg ataaagcgga attaattccc gagcctccaa aaaagaagag aaaggtcgaa
     3541 ttgggatcca cgccgatgga gttccagtac ctgcccgata cggatgaccg tcaccgtatc
     3601 gaagaaaagc ggaagcgaac ctatgaaacc ttcaagtcca tcatgaaaaa gtcccccttc
     3661 tcgggcccca cggacccgcg ccccccgccc cgtcgtattg cggttccttc gcgcagcagt
     3721 gccagcgtcc ccaaacccgc accgcagccc tacccgttca cttcctccct tagcacgatt
     3781 aactatgatg agttccccac gatggtgttc cccagtggac aaatttccca ggcatcggca
     3841 ctggctccgg ccccaccgca agtcctcccc caggcgcccg ctccggcacc ggctcccgca
     3901 atggtgagtg ctctggccca ggcccccgct ccagtccccg tgctggcgcc tggaccccca
     3961 caggcagttg cccctcctgc tccgaaacca acgcaggcgg gcgaaggaac cctgagcgag
     4021 gccctcttgc agcttcagtt cgatgacgaa gacttgggag ccctgctggg taacagcaca
     4081 gaccctgccg tattcaccga tctcgcatcc gtggacaaca gcgagtttca gcagctcttg
     4141 aatcagggaa tcccggtcgc acctcatacc acagagccca tgctgatgga atacccggag
     4201 gctatcacgc gactggtgac cggcgcacag cgaccacccg atccagcccc tgccccactg
     4261 ggtgccccgg gtttgcccaa tggcctcctc agcggcgatg aggatttctc cagcatcgct
     4321 gatatggatt tctccgcttt gctgagccag ataagctccg ctagcggagg aggtggtgga
     4381 ggtggaggtg gaggtactag tgaccaactg actgaagagc agatcgcaga atttaaagag
     4441 gctttctccc tatttgacaa ggacggggat gggacaataa caaccaagga gctggggacg
     4501 gtgatgcggt ctctggggca gaaccccaca gaagcagagc tgcaggacat gatcaatgaa
     4561 gtagatgccg acggtgacgg cacaatcgac ttccctgagt tcctgacaat gatggcaaga
     4621 aaaatgaaag acacagacag tgaagaagaa attagagaag cgttccgtgt gtttgataag
     4681 gatggcaatg gctacatcag tgcagcagag cttcgccacg tgatgacaaa ccttggagag
     4741 aagttaacag atgaagaggt tgatgaaatg atcagggaag cagacatcga tggggatggt
     4801 caggtaaact acgaagagtt tgtacaaatg atgacagcga agtaactcga gggtacctct
     4861 agaggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     4921 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     4981 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     5041 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     5101 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     5161 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     5221 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     5281 aaattatgga aaaatatttg atgtatagtg ccttgactag agatcataat cagccatacc
     5341 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     5401 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     5461 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5521 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcggatccac tagaaggcct
     5581 tagtatgtat gtaagttaat aaaacccttt tttggagaat gtagatttaa aaaaacatat
     5641 ttttttttta ttttttactg cactggacat cattgaactt atctgatcag ttttaaattt
     5701 acttcgatcc aagggtattt gaagtaccag gttctttcga ttacctctca ctcaaaatga
     5761 cattccactc aaagtcagcg ctgtttgcct ccttctctgt ccacagaaat atcgccgtct
     5821 ctttcgccgc tgcgtccgct atctctttcg ccaccgtttg tagcgttacc tagcgtcaat
     5881 gtccgccttc agttgcactt tgtcagcggt ttcgtgacga agctccaagc ggtttacgcc
     5941 atcaattaaa cacaaagtgc tgtgccaaaa ctcctctcgc ttcttatttt tgtttgtttt
     6001 ttgagtgatt ggggtggtga ttggttttgg gtgggtaagc aggggaaagt gtgaaaaatc
     6061 ccggcaatgg gccaagagga tcaggagcta ttaattcgcg gaggcagcaa acacccatct
     6121 gccgagcatc tgaacaatgt gagtagtaca tgtgcataca tcttaagttc acttgatcta
     6181 taggaactgc gattgcaaca tcaaattgtc tgcggcgtga gaactgcgac ccacaaaaat
     6241 cccaaaccgc aatcgcacaa acaaatagtg acacgaaaca gattattctg gtagctgtgc
     6301 tcgctatata agacaatttt taagatcata tcatgatcaa gacatctaaa ggcattcatt
     6361 ttcgactaca ttctttttta caaaaaatat aacaaccaga tattttaagc tgatcctaga
     6421 tgcacaaaaa ataaataaaa gtataaacct acttcgtagg atacttcgtt ttgttcgggg
     6481 ttagatgagc ataacgcttg tagttgatat ttgagatccc ctatcattgc agggtgacag
     6541 cggagcggct tcgcagagct gcattaacca gggcttcggg caggccaaaa actacggcac
     6601 gctcctgcca cccagtccgc cggaggactc cggttcaggg agcggccaac tagccgagaa
     6661 cctcacctat gcctggcaca atatggacat ctttggggcg gtcaatcagc cgggctccgg
     6721 atggcggcag ctggtcaacc ggacacgcgg actattctgc aacgagcgac acataccggc
     6781 gcccaggaaa catttgctca agaacggtga gtttctattc gcagtcggct gatctgtgtg
     6841 aaatcttaat aaagggtcca attaccaatt tgaaactcag tttgcggcgt ggcctatccg
     6901 ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa agacgaccct gctgaatgcc
     6961 cttgcctttc gatcgccgca gggcatccaa gtatcgccat ccgggatgcg actgctcaat
     7021 ggccaacctg tggacgccaa ggagatgcag gccaggtgcg cctatgtcca gcaggatgac
     7081 ctctttatcg gctccctaac ggccagggaa cacctgattt tccaggccat ggtgcggatg
     7141 ccacgacatc tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt
     7201 tcgctcagca aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc
     7261 ggcggagaaa ggaagcgtct ggcattcgcc tccgaggcac taaccgatcc gccgcttctg
     7321 atctgcgatg agcccacctc cggactggac tcatttaccg cccacagcgt cgtccaggtg
     7381 ctgaagaagc tgtcgcagaa gggcaagacc gtcatcctga ccattcatca gccgtcttcc
     7441 gagctgtttg agctctttga caagatcctt ctgatggccg agggcagggt agctttcttg
     7501 ggcactccca gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg
     7561 tatctagcat tacattacat ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa
     7621 ctacaatccg gcggactttt acgtacaggt gttggccgtt gtgcccggac gggagatcga
     7681 gtcccgtgat cggatcgcca agatatgcga caattttgct attagcaaag tagcccggga
     7741 tatggagcag ttgttggcca ccaaaaattt ggagaagcca ctggagcagc cggagaatgg
     7801 gtacacctac aaggccacct ggttcatgca gttccgggcg gtcctgtggc gatcctggct
     7861 gtcggtgctc aaggaaccac tcctcgtaaa agtgcgactt attcagacaa cggtgagtgg
     7921 ttccagtgga aacaaatgat ataacgctta caattcttgg aaacaaattc gctagatttt
     7981 agttagaatt gcctgattcc acacccttct tagttttttt caatgagatg tatagtttat
     8041 agttttgcag aaaataaata aatttcattt aactcgcgaa catgttgaag atatgaatat
     8101 taatgagatg cgagtaacat tttaatttgc agatggttgc catcttgatt ggcctcatct
     8161 ttttgggcca acaactcacg caagtgggcg tgatgaatat caacggagcc atcttcctct
     8221 tcctgaccaa catgaccttt caaaacgtct ttgccacgat aaatgtaagt cttgtttaga
     8281 atacatttgc atattaataa tttactaact ttctaatgaa tcgattcgat ttaggtgttc
     8341 acctcagagc tgccagtttt tatgagggag gcccgaagtc gactttatcg ctgtgacaca
     8401 tactttctgg gcaaaacgat tgccgaatta ccgctttttc tcacagtgcc actggtcttc
     8461 acggcgattg cctatccgat gatcggactg cgggccggag tgctgcactt cttcaactgc
     8521 ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct tcggatatct aatatcctgc
     8581 gccagctcct cgacctcgat ggcgctgtct gtgggtccgc cggttatcat accattcctg
     8641 ctctttggcg gcttcttctt gaactcgggc tcggtgccag tatacctcaa atggttgtcg
     8701 tacctctcat ggttccgtta cgccaacgag ggtctgctga ttaaccaatg ggcggacgtg
     8761 gagccgggcg aaattagctg cacatcgtcg aacaccacgt gccccagttc gggcaaggtc
     8821 atcctggaga cgcttaactt ctccgccgcc gatctgccgc tggactacgt gggtctggcc
     8881 attctcatcg tgagcttccg ggtgctcgca tatctggctc taagacttcg ggcccgacgc
     8941 aaggagtagc cgacatatat ccgaaataac tgcttgtttt tttttttacc attattacca
     9001 tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg tgccaataaa
     9061 aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca caagtagact
     9121 ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc aaaaactcgt
     9181 ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca aatcgcgcgc
     9241 cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg ctgtctccca
     9301 cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact gtcgttagtt
     9361 ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca atagaaatta
     9421 gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat atttccctca
     9481 cagctatatt tattctaatt taatattatg actttttaag gtaatttttt gtgacctgtt
     9541 cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt tccttgtgta
     9601 gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca aaaataacaa
     9661 ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg aactagaatt
     9721 aattcggctg ctgctctaaa cgacgcattt cgtactccaa agtacgaatt ttttccctca
     9781 agctcttatt ttcattaaac aatgaacagg acctaacgca cagtcacgtt attgtttaca
     9841 taaatgattt tttttactat tcaaacttac tctgtttgtg tactcccact ggtatagcct
     9901 tcttttatct tttctggttc aggctctatc actttactag gtacggcatc tgcgttgagt
     9961 cgcctccttt taaatgtctg accttttgca ggtgcagcct tccactgcga atcattaaag
    10021 tgggtatcac aaatttggga gttttcacca aggctgcacc caaggctctg ctcccacaat
    10081 tttctcttaa tagcacactt cggcacgtga attaatttta ctccagtcac agctttgcag
    10141 caaaatttgc aatatttcat ttttttttat tccacgtaag ggttaatgtt ttcaaaaaaa
    10201 aattcgtccg cacacaacct ttcctctcaa caagcaaacg tgcactgaat ttaagtgtat
    10261 acttcggtaa gcttcggcta tcgacgggac caccttatgt tatttcatca tg
//
