LOCUS       sequence-109042-       10365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  KIF1A aa 391-1698 with N-terminal FRB and triple myc tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10365)
  AUTHORS   Bentley M, Decker H, Luisi J, Banker G
  TITLE     A novel assay reveals preferential binding between Rabs, kinesins, 
            and specific endosomal subpopulations.
  JOURNAL   J Cell Biol. 2015 Feb 2;208(3):273-281. Epub 2015 Jan 26.
  PUBMED    25624392
REFERENCE   2  (bases 1 to 10365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2015-02-2"; volume: "208(3)"; pages: "273-281. Epub 
            2015 Jan 26"
COMMENT     Sequence Label: pBa-FRB-3myc-KIF1A tail 391-1698
FEATURES             Location/Qualifiers
     source          1..10365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        418..693
                     /label=chicken beta-actin promoter
     primer_bind     1626..1648
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1728..2006
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     CDS             5532..5543
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    6202..6336
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6239..6258)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6293..6312
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6368..6384)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6368..6384)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6381..6403)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6392..6408
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6416..6422,6423..6440,6441..6446))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6461..6482
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6599..6616)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6770..7358)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6850..6869)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7529..8320,8321..8389))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8152..8171
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8390..8494)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8562..8580
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8618..8640)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8740..8759
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8953..8975
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8967..8984
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8968..8984
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttagtgcatg catacccctc ggtgttattg ctgctctgtg cacgcacgct cattgtatca
       61 cttcatccca gtgcatgcac tcacactgga gcgattgctg ctcggtgcac gcacactcat
      121 tgtatcacgt cagctcagtg gctgcacgca caccggtgtt attgctgctc ggtgcgtgca
      181 tgcacatcag tgtcgctgca gctcagtgca tgcacgctca ttgcccatcg ctatccctgc
      241 ctctcctgct ggcgctcccc gggaggtgac ttcaagggga ccgcaggacc acctcggggg
      301 tggggggagg gctgcacacg cggaccccgc tccccctccc caacaaagca ctgtggaatc
      361 aaaaaggggg gaggggggat ggaggggcgc gtcacacccc cgccccacac cctcacctcg
      421 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
      481 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc
      541 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
      601 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
      661 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt
      721 gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
      781 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
      841 cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg
      901 tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
      961 cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     1021 cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga
     1081 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     1141 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     1201 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     1261 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     1321 aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     1381 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag
     1441 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg
     1501 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     1561 tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag
     1621 ggcggggttc ggcttctggc gtgtgaccgg cggggtttat atcttccctt ctctgttcct
     1681 ccgcagcccc caagcttggc gcgcctaggc ctaccatggc ttctagaatc ctctggcatg
     1741 agatgtggca tgaaggcctg gaagaggcat ctcgtttgta ctttggggaa aggaacgtga
     1801 aaggcatgtt tgaggtgctg gagcccttgc atgctatgat ggaacggggc ccccagactc
     1861 tgaaggaaac atcctttaat caggcctatg gtcgagattt aatggaggcc caagagtggt
     1921 gcaggaagta catgaaatca gggaatgtca aggacctcct ccaagcctgg gacctctatt
     1981 atcatgtgtt ccgacgaatc tcaaagacta gttatccgta cggaatgcag aagctgatct
     2041 cagaggagga cctgcaaaaa cttatttctg aagaggatct gcaaaagctt atttctgaag
     2101 aagatctgta catagatatc actgacacca acactgtgcc tggaggaccc aaattgacca
     2161 atgccctggt gggcatgagc ccctcgtctt cgctctcagc cctgtccagc cgtgcggcct
     2221 ctgtgtccag tctccacgag cgcatcttgt ttgctccagg cagcgaggag gctattgaaa
     2281 ggctgaagga aacagagaag atcattgctg agctcaatga gacgtgggag gagaagctac
     2341 ggcggacaga agccatccgg atggagaggg aagccctgct ggccgagatg ggtgtggcca
     2401 tgagggaaga tggtggcacc ttgggagtgt tttctcccaa aaagacacct catcttgtca
     2461 acctgaacga ggatcctttg atgtctgaat gcctccttta ctacatcaaa gatggcgtca
     2521 ccagagtggg cagagaagac gcagagaggc ggcaagacat tgtcctgagt gggcacttca
     2581 tcaaggagga acactgcatc ttccggagcg actcccgagg aggcggagaa gctgtggtga
     2641 ccctggagcc ctgtgaagga gcagatacct atgtgaacgg caagaaagtc actgagccca
     2701 gcatcctacg gtcaggaaac cgcatcatca tgggaaaaag ccacgtgttc cggtttaacc
     2761 accccgagca ggctcgtcag gagcgtgagc ggacaccttg tgcagagaca cccgctgagc
     2821 cagtggactg ggcctttgcc cagagggagc tgctggagaa gcagggcatt gacatgaagc
     2881 aggagatgga gcagaggctc caggagctgg aggaccagta ccggagggag cgggaggagg
     2941 ccacatacct gctggagcag cagaggctgg actatgagag taagctggag gccctacaga
     3001 aacagatgga ctccaggtac tacccagagg tgaacgagga ggaggaagaa ccagaggatg
     3061 aagtccaatg gacggagcgg gagtgtgagt tggcactgtg ggccttccgg aaatggaagt
     3121 ggtaccagtt cacttctctt cgggacctgc tgtggggcaa tgccatcttc ctgaaggagg
     3181 ccaatgccat cagtgtggag ctaaagaaga aggtccagtt ccagttcgtc ctcctcacag
     3241 ataccctcta ctccccactg ccgccagacc tactcccccc agaagctgcc aaagaccgag
     3301 agacacgacc cttccccagg accatcgtgg ccgtggaggt ccaggaccag aagaatgggg
     3361 ccacccacta ctggacgcta gagaaactca ggcaacgcct ggacctgatg cgggaaatgt
     3421 atgaccgggc tgcagaggtc ccctccagcg tcgttgagga ctgtgacaat gtggtgactg
     3481 gtggagaccc cttctatgac cgcttcccct ggttccggct ggtgggcagg gccttcgtat
     3541 acctgagcaa cttgctgtac cctgtgcccc tggtgcaccg cgtggccatc gtcagcgaaa
     3601 agggagaggt gaaaggcttc ctccgtgtgg cggtgcaggc catctcagct gatgaggagg
     3661 ctccggatta tggatcaggt gtccgtcagt caggaacggc taaaatctcc ttcgatgacc
     3721 agcactttga aaagttccag tctgagtcct gccctgtggt ggggatgtcc cgctcaggga
     3781 cctcccagga ggagctgcgc attgtagaag gccagggtca gggggctgat gctgggcctt
     3841 cagccgatga agtgaacaac aacacttgct cagctgtgcc tcctgaaggc ctcctagata
     3901 gcccagagaa ggctgccctg gatggacccc tagacacagc cctggaccac ctccgtctgg
     3961 gcagtacctt caccttccgg gttacagtcc tgcaggcgtc cagtatctct gctgaatacg
     4021 ctgacatctt ctgccagttc aactttatcc accgccacga tgaggccttc tccactgaac
     4081 ccctgaagaa cactggcaga ggaccccctc ttggcttcta ccatgtccaa aatattgcgg
     4141 tagaggtgac caagtccttc atcgaataca tcaagagcca gcccattgtg ttcgaggtct
     4201 ttgggcacta ccagcagcac ccgttcccac ctctttgcaa ggatgtcctc agccccctga
     4261 gaccctcacg tcgccacttc cccagggtca tgccgttatc caagccagtg cccgccacta
     4321 agctcagcac gatgacacgg ccttccccag gtccctgcca ttgcaagtat gacctgctgg
     4381 tctacttcga gatctgcgag ctggaagcca atggcgatta catccctgct gtggtggacc
     4441 accgtggtgg catgccgtgc atggggactt tccttctcca tcagggcatc cagagacgga
     4501 taactgtgac actgttacat gagacaggaa gccacatccg ctggaaggaa gtgcgcgagc
     4561 tagttgtggg tcgcatccga aacaccccag aaactgacga agctctgatt gaccccaaca
     4621 tcttgtccct caacatcctc tcatcaggat acgtccatcc agcccaggat gaccggcagt
     4681 ttcttgattc ggacatacct aggaccttct accagtttga agcagcctgg gacagttcca
     4741 tgcataactc tctcctgctg aatcgtgtca ccccttaccg ggagaaaatc tacatgacgc
     4801 tctctgccta cattgagatg gagaactgta cccagccagc tgtcatcacc aaggacttct
     4861 gcatggtttt ctactctcgg gacgccaagc tacccgcctc acgctccatt cgcaacctgt
     4921 ttggcagtgg cagcctgcgg gccacagaag gcaaccgtgt gactggtgtg tatgagctca
     4981 gcctttgcca cgtggccgac gcaggcagcc cagggatgca gcgtcggcgc cggcgggtgc
     5041 tggacacatc agtagcctat gtccggggtg aggagaacct ggctggctgg aggccccgaa
     5101 gtgacagcct gatcctagat caccagtggg aactggagaa actgagcctc ttacaggagg
     5161 tggaaaagac caggcactac ctgctccttc gggagaagct ggagacgacc cagcgccctg
     5221 gccctgaggt gctgtcccca gcctccagtg aggactctga gtcccgtagt tcttcaggtg
     5281 cctcttcccc attgtcagcc gagggtcagc cgtcacctct ggaggctccc aacgagaggc
     5341 agagggagct ggctgtcaag tgcttacgac tcctcatgca cacattcaac agggaataca
     5401 cccacagcca cgtctgtatc agcgccagcg agagcaagct ctctgagatg tctgtcaccc
     5461 tgatgcggga cccatccatg tcccctctgg gtgctgctac actcactccc tcctccactt
     5521 gcccctctct gatcgaagga cgatacggtg ccactgacgt gaggaccccc cagccctgct
     5581 cccggcccgc cagcccagag cccgagctcc tgccagaact tgactctaag aagactccct
     5641 cccctgttcg ggcaacagag acagagaagg agccccagcg actgctggtc cctgacatcc
     5701 aagagatccg agtcagccca atcgtctcca agaagggata cctgcatttc ctggagccac
     5761 acacggctgg ctgggccaag cgctttgtgg tggtgaggcg tccctacgcc tacatgtaca
     5821 acagtgacaa ggacacggtg gagagatttg tgctcaacct ctccactgcg caggtggagt
     5881 acagcgagga ccagcaggcc atgctcaaga cacccaacac attcgctgtg tgcacggagc
     5941 accgaggcat cctgctgcag gccaacagtg acaaggacat gcacgactgg ctgtatgcct
     6001 tcaacccgct cctggctggg accatccggt ccaagctctc tcgaaggaga tctgcgcaga
     6061 tgcgggtctg agttgaaagc cttccggaat tcattgatca taatcagcca taccacattt
     6121 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     6181 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     6241 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6301 tccaaactca tcaatgtatc ttatcatgtc tggatccccg ggtaccgagc tcgaattcgt
     6361 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6421 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6481 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6541 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6601 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6661 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6721 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6781 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6841 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6901 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6961 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7021 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7081 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7141 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7201 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7261 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7321 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7381 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7441 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7501 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7561 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7621 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7681 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7741 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7801 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     7861 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     7921 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     7981 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8041 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8101 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8161 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8221 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8281 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8341 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8401 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8461 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8521 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     8581 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     8641 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     8701 cagcgggtgt tggcgggtgt cggggttggc ttaactatgc ggcatcagag cagattgtac
     8761 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     8821 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
     8881 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     8941 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ttgggatctt
     9001 tgcattggcc cacggctctc aggatgggga tgctcccctt cagcacccgg ttccccttgg
     9061 aaactgatgg tcctggctct gtggcatggc agtggcactg tgaggagccc ctaccagcag
     9121 cacacagtgg gtttggcact gccacgctcc ggatgccgcg ctctgatcca accccataat
     9181 caagggaacc cgaattgccc catcattgcc cccaccaccc ccatcctgcc gggccctcac
     9241 accccacgct gccttgtggt gacattcccc agcccaaacc cacggcttca tggctaccgc
     9301 ggggcatttc ccattgccgc cccattatca gctctgcaca cctcccgctg tacccatgcc
     9361 tcgtggctgc ccttctttga cgtataatct tctaattaat acccggcctt gtcaaagtgg
     9421 agcacaaacg ttaattaatt ccccagcagg caggtaatta acagtgtgac tccctttttg
     9481 ctgcgagtgg ggctgataca gagagatgtg gcactatgga gcccacgggg tcctggcact
     9541 gggtgcccac ggaggtcccc atgtgctgca gtgtcaccgc ctccgaggtg acagtattgt
     9601 ccctgcggtg tccctgcagc tcagctctgt ccacagggcc acctccagtt tggaggggac
     9661 acaatgcagc cccgatgcaa cccatcctcg cagcatccca gggacaaaga ccccactgca
     9721 agaccgcaca cagggctggg tcccgctccc ctaatatcta cagtgctttt gcatggcccc
     9781 ttaatcaatg cagttaatca gcatgcgctc atgcaccgct ctggagctgc aaagcccctc
     9841 gcagcgctgc tcaccaacac cgcgcaccgc cccggcccag cctgcagcac gcgctgcaaa
     9901 caggaaagaa acaaaatatt gcccaaatgt aggcaaaggc attcggctgc cttgacctcc
     9961 gccgggccgg gccctgcctg actcagctcc ttactcagcg ctcgcttcct ccctccggct
    10021 gccaccgccg cagcgcacac cctgacaaag agtggccctt aacgggctct gaggtgcacc
    10081 cagcagtgca ctcagcagtc caagggccgg cctggaggtt tgcaccgcta cgtgctgaca
    10141 ttagcattga acttggccct gggtagtgct gcaggccggg cggggtgggt gtagagagtg
    10201 cagcgcgcgt tgcacccggt gccccttccc ctcccttgca tcccagcagg ctgcacccca
    10261 gcaccaggcc cgtgcatgca tgctcctggt gttattgcag cctggtgcat gcatgcgtct
    10321 tagtggtgca gcgctgtgca tgcatcctcc ttggtgtgta gcagc
//
