LOCUS       sequence-109045-       10625 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  KIF13A aa 361-1749 with N-terminal FRB and triple myc tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10625)
  AUTHORS   Bentley M, Decker H, Luisi J, Banker G
  TITLE     A novel assay reveals preferential binding between Rabs, kinesins, 
            and specific endosomal subpopulations.
  JOURNAL   J Cell Biol. 2015 Feb 2;208(3):273-281. Epub 2015 Jan 26.
  PUBMED    25624392
REFERENCE   2  (bases 1 to 10625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2015-02-2"; volume: "208(3)"; pages: "273-281. Epub 
            2015 Jan 26"
COMMENT     Sequence Label: pBa-FRB-3myc-KIF13A tail 361-1749
FEATURES             Location/Qualifiers
     source          1..10625
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        418..693
                     /label=chicken beta-actin promoter
     primer_bind     1626..1648
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1728..2006
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     misc_feature    2120..2176
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      4842..4851
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    6462..6596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6499..6518)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6553..6572
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6628..6644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6628..6644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6641..6663)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6652..6668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6676..6682,6683..6700,6701..6706))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6721..6742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6859..6876)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7030..7618)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7110..7129)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7789..8580,8581..8649))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8412..8431
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8650..8754)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8822..8840
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8878..8900)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9000..9019
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9213..9235
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9227..9244
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9228..9244
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttagtgcatg catacccctc ggtgttattg ctgctctgtg cacgcacgct cattgtatca
       61 cttcatccca gtgcatgcac tcacactgga gcgattgctg ctcggtgcac gcacactcat
      121 tgtatcacgt cagctcagtg gctgcacgca caccggtgtt attgctgctc ggtgcgtgca
      181 tgcacatcag tgtcgctgca gctcagtgca tgcacgctca ttgcccatcg ctatccctgc
      241 ctctcctgct ggcgctcccc gggaggtgac ttcaagggga ccgcaggacc acctcggggg
      301 tggggggagg gctgcacacg cggaccccgc tccccctccc caacaaagca ctgtggaatc
      361 aaaaaggggg gaggggggat ggaggggcgc gtcacacccc cgccccacac cctcacctcg
      421 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
      481 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc
      541 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
      601 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
      661 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt
      721 gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
      781 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
      841 cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg
      901 tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
      961 cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     1021 cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga
     1081 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     1141 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     1201 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     1261 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     1321 aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     1381 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag
     1441 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg
     1501 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     1561 tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag
     1621 ggcggggttc ggcttctggc gtgtgaccgg cggggtttat atcttccctt ctctgttcct
     1681 ccgcagcccc caagcttggc gcgcctaggc ctaccatggc ttctagaatc ctctggcatg
     1741 agatgtggca tgaaggcctg gaagaggcat ctcgtttgta ctttggggaa aggaacgtga
     1801 aaggcatgtt tgaggtgctg gagcccttgc atgctatgat ggaacggggc ccccagactc
     1861 tgaaggaaac atcctttaat caggcctatg gtcgagattt aatggaggcc caagagtggt
     1921 gcaggaagta catgaaatca gggaatgtca aggacctcct ccaagcctgg gacctctatt
     1981 atcatgtgtt ccgacgaatc tcaaagacta gttatccgta cggaatgcag aagctgatct
     2041 cagaggagga cctgcaaaaa cttatttctg aagaggatct gcaaaagctt atttctgaag
     2101 aagatctgta caagtactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac
     2161 cgcgggcccg ggatcccaat gccaaggtca tccgagagct tcgagaggaa gtggagaaac
     2221 tgagggagca gctctcgcag gctgaggcca tgaaagcccc cgagctaaag gagaagctcg
     2281 aagagtctga gaagctgata aaggagctaa cggtgacgtg ggaagagaag ctgaggaaaa
     2341 cagaagcgat cgcacaggag agacagcggc agctggaaag catgggaatc tccctggaga
     2401 cctctggcat caaggtgggg gacgacaagt gctacctcgt caacctgaac gcagaccctg
     2461 ccctcaacga gcttctggtt tactatttaa aggatcatac ccgggtgggt gcagatacct
     2521 ctcaggacat ccagctcttc ggaataggga ttcagcccga gcactgtgag atcgacatcg
     2581 cagcggatgg agacatcact ctcacgccca aagaaaatgc aaggtcctgt ggcaatggca
     2641 cccttgtctg cagtactacc cagctgtggc acggcgacag gatcttatgg ggaaacaacc
     2701 acttttttag aattaacttg cctaagagga aacggcggga ctggttgaaa gactttgaga
     2761 gagaaaccag ttccgcggag cacgatctag acgctgcgag cgaggcttcc tcggagccag
     2821 actacaacta cgagttcgca cagatggagg tcatcatgaa aacactgaac agcaatgatc
     2881 cagttcaaaa tgtggttcag gtcctggaga agcagtacct ggaagagaag cggaccgccc
     2941 tggaggaaca gcggctcatg tacgaacgcg agctggagca gcttcgccag cagctctctc
     3001 ccgagaggca gccgcccagc agcgcctcag accgtctggc ctacagcagc cagacagccc
     3061 agcagaaggt gacccagtgg gccgaggaga gggatgaact ctttaggcag agcttggcca
     3121 agcttcgaga gcagctggtg aaagccaaca ctttggtgag ggaagccaac ttcctagccg
     3181 aggagatgag caaactcact gactaccaag tgactctgca gatccctgcg gccaacctca
     3241 gtgccaacag gaagagaggc gcaatcgtca gcgagcccgc catccaagcc aggaggaaag
     3301 ggaagggcac ccaggtctgg accattgaga agctggagaa taagctaatt gatatgagag
     3361 acctttacca agaatggaaa gaaaatgttc ctgaggccaa gaggctctat gggaagcgag
     3421 gtgacccttt ttatgaagcg caggagaatc acaacctcat tggtgtggct aatgtgtttt
     3481 tggaatgtct cttctgtgat gtgaagcttc aatatgcagt acccatcatc agccagcagg
     3541 gggaggttgc cgggcgcctc catgtggagg tgacgcgcat tacaggaacc attccagaac
     3601 gcatggcgga ggatgactct tcagagaact ctagtgagag cgggagtctt gaagtggtcg
     3661 acagcagcgg cgaagtcatt caccgggtca aaaagctgac atgcagggtg ataattaaag
     3721 aggcaaccgg gctacccata agcctctcaa atttcgtctt ctgtcagtac acgttctggg
     3781 accagtgtga gtctacagtg gctgctccag tggtggaccc agatgtgccc tccccgcagt
     3841 ccaaggacgc acagtacacg gtgaccttct ctcactgtaa ggactacgtg gtgactgtca
     3901 cagaagagtt cctggagttc atttctgatg gtgcgctggc gattgaagtg tggggccaca
     3961 ggtgtgctgg gaacggaagc cccatctggg aggtggactc gctccatgcg aagacaagga
     4021 cattgcatga caggtggaat gaagtgaccc ggagaataga ggtgtggatc tccattctgg
     4081 agctgaatga gctcggggat tatgccgctg tggagcttca ccaggcgaaa gacgtcaaca
     4141 ccggcggcgt cttccaactc agacagggtc actcccgtag agtgcaggtc acagtgaaac
     4201 ctgtacagca ctcagggaca ctgccactca tggttgaagc catcctatca gtatccattg
     4261 gctgcgtgac cgccaggtcc accaagctgc agagagggct ggacagctac caggaagaag
     4321 acttgaattg tgtaagagag agatggtccg atgcactcat taaacgacgg gaatacttag
     4381 acgaacagat taaaaaagtc agcaataaaa aagagaaaac agaagacgac atggagcggg
     4441 aagccaggct ggtggagcag tgggtggggc tgacagagga gaggaatgct gtgctggtgc
     4501 ccgcccctgg cagcggaatc cccggggcac ctgctgactg ggtcccacct cctggcatgg
     4561 aaacacacat cccggttctc ttcctggatc tgaacgcgga tgacctcagt gccaatgagc
     4621 agctggtggg cccccatgcg tccggtgtga actccatcct gcccaaggaa cacggcagtc
     4681 agtttttcta cctgcccatc ataaagcaca gtgatgacga ggtttcagcc acagcctcct
     4741 gggactcctc tgtgcacgac tctcttcacc tgaatcgggt cacaccgcag aatgaaagga
     4801 tctacctgat cgtgaagacc accgtgcagc tcagccaccc ggccgccatg gagctagtgt
     4861 tgcggaagcg gatcgcggcc aatatttata acaagcagag tttcacccag agtttgaaga
     4921 gacgaatatc actgatcaat atacgttact cctgcggcgt gacctatgag atagtatcca
     4981 atatcccaaa ggcgaccgag gagattgagg accgggaaac cctggccctc ctggcagcgc
     5041 ggagtgagaa cgagggcact ttggatgggg agacatacat cgagaagtac acgcggggcg
     5101 tgctgcaggt ggagaatatc ctgagcctgg agcggctccg gcaggctgtc acggtgaagg
     5161 aagcgctttc caccaaagcg aggcacattc ggagaagcct cagtacaccg aacgttcaca
     5221 acgtctcctc tagccggcca gatctctctg gcttcgatga agacgataag ggctggcccg
     5281 agaaccagtt agacgtgtct gactacagct ccagctacca agacgtcgcg tgctatggga
     5341 ctttacccag ggactcacct cgcaggagca aggaaggttg tccatcagag aatcctcatg
     5401 ccttaacagt cagccctttt aaagcgttct ctcctcagcc accaaagttc ttcaaacccc
     5461 tcatgcctgt aaaagaggag cataagaaaa ggttggccct ggaagccaga cctctcctaa
     5521 gccaagagga ctcggaagag gaggaaaacg agctggaagc tctcagcagg aagctgatgc
     5581 ttacacagcc ttatgtgccc gtggagttcg ctgacttcag tgtttacaat gccagcttgg
     5641 agaacaggga gtggtcctct tctaaggcgg acctgacaga ctcgagagcc ttggagaaag
     5701 cagtgtcccg tagccctacc accagcagcc tcaccagtgg ctacttctcc cacagtgcct
     5761 ccaatgccac tctgtctgac atggcagtcc cctccagcga cagctcagac cagcttgccg
     5821 tctcgacaaa ggaggtcgag tgcgccgagc ccccgggacc atcacttgca ccggatgtca
     5881 gacgagcttc gaatcaggag cttacagaag taggaagagg ctcagggaag gatgagacaa
     5941 tcgctgtgcc gctggaggaa aacagtgcct tacccaaggg gaccccgtca ccccagagca
     6001 tccctgagga aagttccaga atgccatgca ggactgcttc atgttcagaa ctggatgtcg
     6061 gccccagcaa agatggccac caagccagag aattctgccc aggagaggtg acgatagaac
     6121 acactacaaa cattttggag gaccattcct tcacagagtt catgggtgtt tctgatggca
     6181 aagactttga tggtttggca gattgttctg taggggagcc ctccaggagg agggctctaa
     6241 caaacgaaac agaccataaa ggtatcccag aaagacctcc agatgccgac cggctgcatc
     6301 ccaagataga gaacgaccag gaagccacag ccactcggta agctagataa gtaatgatca
     6361 taatcagcca tatcacatct gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
     6421 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
     6481 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     6541 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccccg
     6601 ggtaccgagc tcgaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6661 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6721 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6781 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6841 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6901 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6961 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7021 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7081 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7141 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7201 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7261 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7321 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7381 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7441 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     7501 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7561 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7621 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7681 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7741 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7801 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7861 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7921 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7981 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8041 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8101 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8161 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8221 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8281 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8341 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8401 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8461 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8521 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8581 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8641 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8701 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8761 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     8821 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     8881 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     8941 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggttggc ttaactatgc
     9001 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
     9061 cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca actgttggga
     9121 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     9181 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     9241 cagtgccaag ttgggatctt tgcattggcc cacggctctc aggatgggga tgctcccctt
     9301 cagcacccgg ttccccttgg aaactgatgg tcctggctct gtggcatggc agtggcactg
     9361 tgaggagccc ctaccagcag cacacagtgg gtttggcact gccacgctcc ggatgccgcg
     9421 ctctgatcca accccataat caagggaacc cgaattgccc catcattgcc cccaccaccc
     9481 ccatcctgcc gggccctcac accccacgct gccttgtggt gacattcccc agcccaaacc
     9541 cacggcttca tggctaccgc ggggcatttc ccattgccgc cccattatca gctctgcaca
     9601 cctcccgctg tacccatgcc tcgtggctgc ccttctttga cgtataatct tctaattaat
     9661 acccggcctt gtcaaagtgg agcacaaacg ttaattaatt ccccagcagg caggtaatta
     9721 acagtgtgac tccctttttg ctgcgagtgg ggctgataca gagagatgtg gcactatgga
     9781 gcccacgggg tcctggcact gggtgcccac ggaggtcccc atgtgctgca gtgtcaccgc
     9841 ctccgaggtg acagtattgt ccctgcggtg tccctgcagc tcagctctgt ccacagggcc
     9901 acctccagtt tggaggggac acaatgcagc cccgatgcaa cccatcctcg cagcatccca
     9961 gggacaaaga ccccactgca agaccgcaca cagggctggg tcccgctccc ctaatatcta
    10021 cagtgctttt gcatggcccc ttaatcaatg cagttaatca gcatgcgctc atgcaccgct
    10081 ctggagctgc aaagcccctc gcagcgctgc tcaccaacac cgcgcaccgc cccggcccag
    10141 cctgcagcac gcgctgcaaa caggaaagaa acaaaatatt gcccaaatgt aggcaaaggc
    10201 attcggctgc cttgacctcc gccgggccgg gccctgcctg actcagctcc ttactcagcg
    10261 ctcgcttcct ccctccggct gccaccgccg cagcgcacac cctgacaaag agtggccctt
    10321 aacgggctct gaggtgcacc cagcagtgca ctcagcagtc caagggccgg cctggaggtt
    10381 tgcaccgcta cgtgctgaca ttagcattga acttggccct gggtagtgct gcaggccggg
    10441 cggggtgggt gtagagagtg cagcgcgcgt tgcacccggt gccccttccc ctcccttgca
    10501 tcccagcagg ctgcacccca gcaccaggcc cgtgcatgca tgctcctggt gttattgcag
    10561 cctggtgcat gcatgcgtct tagtggtgca gcgctgtgca tgcatcctcc ttggtgtgta
    10621 gcagc
//
