LOCUS       sequence-109052-       10535 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  generating transgenic TRIC flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10535)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 10535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pBP-p65AD::CaM
FEATURES             Location/Qualifiers
     source          1..10535
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     37..55
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    83..207
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(232..237,238..255,256..262)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             316..975
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(382..401)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1317..1622
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1501..1520
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1663..1787)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1997..2017
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2468..2839
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALLSQISS"
     CDS             2882..3325
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAEL
                     RHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     intron          3412..3477
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3607..3627
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    3899..4020
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3936..3955)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3990..4009
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(join(5057..5671,5742..5873,6077..6392,6454..7108,
                     7183..7456,7858..7929))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     8381..8399
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     protein_bind    8624..8693
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     rep_origin      complement(8780..9368)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8860..8879)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9539..10330,10331..10399))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10162..10181
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10400..10504)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
       61 cgtcttcaag aattcgttta tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
      121 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
      181 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
      241 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
      301 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      361 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      421 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      481 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
      541 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      601 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      661 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
      721 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
      781 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
      841 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
      901 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
      961 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     1021 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     1081 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     1141 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     1201 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     1261 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     1321 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     1381 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     1441 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     1501 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     1561 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     1621 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     1681 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     1741 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat aaacggccgg
     1801 ccgagctcgc ccggggatcg agcgcagcgg tataaaaggg cgcggggtgg ctgagagcat
     1861 cagttgtgaa tgaatgttcg agccgagcag acgtgccgct gccttcgtta atatcctttg
     1921 aataagccaa ctttgaatca caagacgcat accaaacggt accaacatgg ataaagcgga
     1981 attaattccc gagcctccaa aaaagaagag aaaggtcgaa ttgggatcca cgccgatgga
     2041 gttccagtac ctgcccgata cggatgaccg tcaccgtatc gaagaaaagc ggaagcgaac
     2101 ctatgaaacc ttcaagtcca tcatgaaaaa gtcccccttc tcgggcccca cggacccgcg
     2161 ccccccgccc cgtcgtattg cggttccttc gcgcagcagt gccagcgtcc ccaaacccgc
     2221 accgcagccc tacccgttca cttcctccct tagcacgatt aactatgatg agttccccac
     2281 gatggtgttc cccagtggac aaatttccca ggcatcggca ctggctccgg ccccaccgca
     2341 agtcctcccc caggcgcccg ctccggcacc ggctcccgca atggtgagtg ctctggccca
     2401 ggcccccgct ccagtccccg tgctggcgcc tggaccccca caggcagttg cccctcctgc
     2461 tccgaaacca acgcaggcgg gcgaaggaac cctgagcgag gccctcttgc agcttcagtt
     2521 cgatgacgaa gacttgggag ccctgctggg taacagcaca gaccctgccg tattcaccga
     2581 tctcgcatcc gtggacaaca gcgagtttca gcagctcttg aatcagggaa tcccggtcgc
     2641 acctcatacc acagagccca tgctgatgga atacccggag gctatcacgc gactggtgac
     2701 cggcgcacag cgaccacccg atccagcccc tgccccactg ggtgccccgg gtttgcccaa
     2761 tggcctcctc agcggcgatg aggatttctc cagcatcgct gatatggatt tctccgcttt
     2821 gctgagccag ataagctccg ctagcggagg aggtggtgga ggtggaggtg gaggtactag
     2881 tgaccaactg actgaagagc agatcgcaga atttaaagag gctttctccc tatttgacaa
     2941 ggacggggat gggacaataa caaccaagga gctggggacg gtgatgcggt ctctggggca
     3001 gaaccccaca gaagcagagc tgcaggacat gatcaatgaa gtagatgccg acggtgacgg
     3061 cacaatcgac ttccctgagt tcctgacaat gatggcaaga aaaatgaaag acacagacag
     3121 tgaagaagaa attagagaag cgttccgtgt gtttgataag gatggcaatg gctacatcag
     3181 tgcagcagag cttcgccacg tgatgacaaa ccttggagag aagttaacag atgaagaggt
     3241 tgatgaaatg atcagggaag cagacatcga tggggatggt caggtaaact acgaagagtt
     3301 tgtacaaatg atgacagcga agtaagcttc ctagggatct ttgtgaagga accttacttc
     3361 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     3421 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     3481 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     3541 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     3601 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     3661 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
     3721 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattt gatgtatagt
     3781 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     3841 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     3901 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     3961 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     4021 tcatgtctac cggtactagt tctagagcgg ccgctggcca cgggtgcgca tgatcgtgct
     4081 cctgtcgttg aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc
     4141 accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac
     4201 aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc
     4261 ctgcaccatt atgttccgga ggcgcgccct agttccagtg aaatccaagc attttctaaa
     4321 ttaaatgtat tcttattatt atagttgtta tttttgatat atataaacaa cactattatg
     4381 cccaccattt ttttgagatg catctacaca aggaacaaac actggatgtc actttcagtt
     4441 caaattgtaa cgctaatcac tccgaacagg tcacaaaaaa ttaccttaaa aagtcataat
     4501 attaaattag aataaatata gctgtgaggg aaatatatac aaatatattg gagcaaataa
     4561 attgtacata caaatattta ttactaattt ctattgagac gaaatgaacc actcggaacc
     4621 atttgagcga accgaatcgc gcggaactaa cgacagtcgc tccaaggtcg tcgaacaaaa
     4681 ggtgaatgtg ttgcggagag cgggtgggag acagcgaaag agcaactacg aaacgtggtg
     4741 tggtggaggt gaattatgaa gagggcgcgc gatttgaaaa gtatgtatat aaaaaatata
     4801 tcccggtgtt ttatgtagcg ataaacgagt ttttgatgta aggtatgcag gtgtgtaagt
     4861 cttttggtta gaagacaaat ccaaagtcta cttgtgggga tgttcgaagg ggaaatactt
     4921 gtattctata ggtcatatct tgtttttatt ggcacaaata taattacatt agctttttga
     4981 gggggcaata aacagtaaac acgatggtaa taatggtaaa aaaaaaaaac aagcagttat
     5041 ttcggatata tgtcggctac tccttgcgtc gggcccgaag tcttagagcc agatatgcga
     5101 gcacccggaa gctcacgatg agaatggcca gacccacgta gtccagcggc agatcggcgg
     5161 cggagaagtt aagcgtctcc aggatgacct tgcccgaact ggggcacgtg gtgttcgacg
     5221 atgtgcagct aatttcgccc ggctccacgt ccgcccattg gttaatcagc agaccctcgt
     5281 tggcgtaacg gaaccatgag aggtacgaca accatttgag gtatactggc accgagcccg
     5341 agttcaagaa gaagccgcca aagagcagga atggtatgat aaccggcgga cccacagaca
     5401 gcgccatcga ggtcgaggag ctggcgcagg atattagata tccgaaggac gttgacacat
     5461 tggccaccag agtgaccagc gccaggcagt tgaagaagtg cagcactccg gcccgcagtc
     5521 cgatcatcgg ataggcaatc gccgtgaaga ccagtggcac tgtgagaaaa agcggcaatt
     5581 cggcaatcgt tttgcccaga aagtatgtgt cacagcgata aagtcgactt cgggcctccc
     5641 tcataaaaac tggcagctct gaggtgaaca cctaaatcga atcgattcat tagaaagtta
     5701 gtaaattatt gaaatgcaaa tgtattctaa acatgactta catttatcgt ggcaaagacg
     5761 ttttgaaagg tcatgttggt caggaagagg aagatggctc cgttgatatt catcacaccc
     5821 acttgcgtga gttgttggcc caaaaagatg aggccaatca agatggcaac catctgcaaa
     5881 ttaaaatgtt actcgcatct cattaatatt cgcgagttaa atgaaattta tttatcttct
     5941 gcaaaactat aaactataca tctcattgaa aaaaactaag aagggtgtgg aatcaggcaa
     6001 ttctatctaa aatctagcga atttgtttcc aagaattgta agcgttatat catttgtttc
     6061 cactggaacc actcaccgtt gtctgaataa gtcgcacttt tacgaggagt ggttccttga
     6121 gcaccgacag ccaggatcgc cacaggaccg cccggaactg catgaaccag gtggccttgt
     6181 aggtgtaccc attctccggc tgctccagtg gcttctccag atttttggtg gccaacaact
     6241 gctccatatc ccgggctact ttgctaatgg caaaattgtc gcatatcttg gcgatccgat
     6301 cacgggactc gatctcccgt ccgggcacaa cggccaacac ctgtacgtaa aagtccgccg
     6361 gattgtagtt ggtaggacac tgggcaccca cgctggatag gagttgagat gttatgtaat
     6421 actagatacc cttaataaac acatcgaact cactaggaaa agaagtcgac ggcttcgctg
     6481 ggagtgccca agaaagctac cctgccctcg gccatcagaa ggatcttgtc aaagagctca
     6541 aacagctcgg aagacggctg atgaatggtc aggatgacgg tcttgccctt ctgcgacagc
     6601 ttcttcagca cctggacgac gctgtgggcg gtaaaggagt ccagtccgga ggtgggctca
     6661 tcgcagatca gaagcggcgg atcggttaga gcctcggagg cgaatgccag acgcttcctt
     6721 tctccgccgg acagaccttt caccctgccg ggcacaccga tgatcgtgtg ctgacatttg
     6781 ctgagcgaaa gctcctggat cacctgatcc acgcgggcca ctcgctgccg ataggtcaga
     6841 tgtcgtggca tccgcaccat ggcttggaaa atcaggtgtt ccctggccgt tagggagccg
     6901 ataaagaggt catcctgctg gacataggcg cacctggcct gcatctcctt ggcgtccaca
     6961 ggttggccat tgagcagtcg catcccggat ggcgatactt ggatgccctg cggcgatcga
     7021 aaggcaaggg cattcagcag ggtcgtcttt ccggcaccgg aactgcccat cacggccaaa
     7081 agttcgcccg gataggccac gccgcaaact gagtttcaaa ttggtaattg gaccctttat
     7141 taagatttca cacagatcag ccgactgcga atagaaactc accgttcttg agcaaatgtt
     7201 tcctgggcgc cggtatgtgt cgctcgttgc agaatagtcc gcgtgtccgg ttgaccagct
     7261 gccgccatcc ggagcccggc tgattgaccg ccccaaagat gtccatattg tgccaggcat
     7321 aggtgaggtt ctcggctagt tggccgctcc ctgaaccgga gtcctccggc ggactgggtg
     7381 gccggagcgt gccgtagttt ttggcctgcc cgaagccctg gttaatgcag ctctgcgaag
     7441 ccgctccgct gtcaccctgc aatgataggg gatctcaaat atcaactact agcgttatgc
     7501 tcatctaacc ccgaacaaaa agtaccccga agtatcctac gaagtaggtt tatactttta
     7561 tttatttttt gtgcatctag gatcagctta aaatatctgg ttgttatatt ttttgtaaaa
     7621 aagaatatag tcgaaaatga atgcctttag atgtcttgat catgatatga tctcaaaaat
     7681 tgtcttatat agcgagaaca gctaccagaa taatctgttt cgtgtcacta tttgtttgtg
     7741 caattgcggt ttgggatttt tgtgggtcgc agttctcacg ccgcatacaa tttgatgttg
     7801 caatcgcagt tcctatagat caagtgaact taagatgtat gcacatgtac tactcacatt
     7861 gttcagatgc tcggcagatg ggtgtttgct gcctccgcga attaatagct cctgatcctc
     7921 ttggcccatt gccgggattt ttcacacttt cccctgctta cccacccaaa accaatcacc
     7981 accccaatca ctcaaaaaac aaacaaaaat aagaagcgag aggagttttg gcacagcact
     8041 ttgtgtttaa ttgatggcgt aaaccgcttg gagcttcgtc acgaaaccgc tgacaaaatg
     8101 caactgaagg cggacattga cgctacgtaa cgctacaaac ggtggcgaaa gagatagcgg
     8161 acgcagcggc gaaagagacg gcgatatttc tgtggacaga gaaggaggca aacagcgctg
     8221 actttgagtg gaatgtcatt ttgagtgaga ggtaatcgaa agaacctggt acatcaaata
     8281 cccttggatc gaagtaaatt taaaactgat cagataagtt caatgatatc cagtgcagta
     8341 aaaaaaaaaa atgttttttt tatctacttt ccgcaaaaat gggttttatt aacttacata
     8401 catactaggc gcgcccatat gttcggcttg tcgacatgcc cgccgtgacc gtcgagaacc
     8461 cgctgacgct gccccgcgta tccgcacccg ccgacgccgt cgcacgtccc gtgctcaccg
     8521 tgaccaccgc gcccagcggt ttcgagggcg agggcttccc ggtgcgccgc gcgttcgccg
     8581 ggatcaacta ccgccacctc gacccgttca tcatgatgga ccagatgggt gaggtggagt
     8641 acgcgcccgg ggagcccaag ggcacgccct ggcacccgca ccgcggcttc gagaccgtga
     8701 cctacatcgt cgacggtaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8761 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8821 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8881 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8941 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     9001 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     9061 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     9121 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     9181 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     9241 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     9301 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     9361 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     9421 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9481 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9541 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9601 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9661 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9721 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9781 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9841 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9901 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9961 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
    10021 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
    10081 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
    10141 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
    10201 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
    10261 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
    10321 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
    10381 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
    10441 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10501 cgcgcacatt tccccgaaaa gtgccacctg acgtc
//
